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Extension of the culture period for the in vitro growth of bovine oocytes in the presence of bone morphogenetic protein-4 increases oocyte diameter, but impairs subsequent developmental competence

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Title: Extension of the culture period for the in vitro growth of bovine oocytes in the presence of bone morphogenetic protein-4 increases oocyte diameter, but impairs subsequent developmental competence
Authors: Yang, Yinghua Browse this author
Kanno, Chihiro Browse this author
Sakaguchi, Kenichiro Browse this author
Yanagawa, Yojiro Browse this author
Katagiri, Seiji Browse this author →KAKEN DB
Nagano, Masashi Browse this author →KAKEN DB
Keywords: BMP-4
bovine oocyte
developmental competence
in vitro growth
Issue Date: Nov-2017
Publisher: Japanese Society of Animal Science
Journal Title: Animal Science Journal
Volume: 88
Issue: 11
Start Page: 1686
End Page: 1691
Publisher DOI: 10.1111/asj.12841
PMID: 28594136
Abstract: Bone morphogenetic protein-4 (BMP-4) inhibits luteinization of granulosa cells during in vitro growth (IVG) culture of bovine oocytes; however, oocytes derived from a 12-day IVG were less competent for development than in vivo-grown oocytes. We herein investigated whether an extended IVG culture with BMP-4 improves oocyte growth and development to blastocysts after in vitro fertilization. Oocyte-granulosa cell complexes (OGCs) were cultured for 14 or 16 days with BMP-4 (10 ng/mL), while a 12-day culture with BMP-4 served as the in vitro control. OGC viability was maintained for the 16-day culture with BMP-4 (83.2%), but was significantly lower without BMP-4 (58.9%) than the control (83.0%). Prolong-cultured oocytes at 16 days had statistically greater diameter (114.6 μm) than the control (111.7 μm). IVG oocytes with BMP-4 for the 16-day culture had a similar nuclear maturation rate to the control (approximately 67%); however, blastocyst rates in BMP-4 treated oocytes of 14- (1.8%) and 16-day (0%) IVG were statistically lower than that of 12-day IVG (9.0%). In conclusion, BMP-4 maintained OGC viability and promoted oocyte growth in a prolonged culture, but impaired the developmental competence of oocytes. Prolonged culture may not be an appropriate strategy for enhancing the developmental competence of IVG oocytes.
Rights: This is the peer reviewed version of the following article: Yang, Y., Kanno, C., Sakaguchi, K., Yanagawa, Y., Katagiri, S., and Nagano, M. (2017) Extension of the culture period for the in vitro growth of bovine oocytes in the presence of bone morphogenetic protein-4 increases oocyte diameter, but impairs subsequent developmental competence. Anim Sci J, 88: 1686–1691. doi: 10.1111/asj.12841., which has been published in final form at https://doi.org/10.1111/asj.12841. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: article (author version)
URI: http://hdl.handle.net/2115/72007
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永野 昌志

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