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Visualising the dynamics of live pancreatic microtumours self-organised through cell-in-cell invasion

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Title: Visualising the dynamics of live pancreatic microtumours self-organised through cell-in-cell invasion
Authors: Miyatake, Yukiko Browse this author →KAKEN DB
Kuribayashi-Shigetomi, Kaori Browse this author
Ohta, Yusuke Browse this author
Ikeshita, Shunji Browse this author
Subagyo, Agus Browse this author
Sueoka, Kazuhisa Browse this author →KAKEN DB
Kakugo, Akira Browse this author →KAKEN DB
Amano, Maho Browse this author
Takahashi, Toshiyuki Browse this author
Okajima, Takaharu Browse this author →KAKEN DB
Kasahara, Masanori Browse this author →KAKEN DB
Issue Date: 19-Sep-2018
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 8
Start Page: 14054
Publisher DOI: 10.1038/s41598-018-32122-w
Abstract: Pancreatic ductal adenocarcinoma (PDAC) reportedly progresses very rapidly through the initial carcinogenesis stages including DNA damage and disordered cell death. However, such oncogenic mechanisms are largely studied through observational diagnostic methods, partly because of a lack of live in vitro tumour imaging techniques. Here we demonstrate a simple live-tumour in vitro imaging technique using micro-patterned plates (micro/nanoplates) that allows dynamic visualisation of PDAC microtumours. When PDAC cells were cultured on a micro/nanoplate overnight, the cells self-organised into non-spheroidal microtumours that were anchored to the micro/nanoplate through cell-in-cell invasion. This self-organisation was only efficiently induced in small-diameter rough microislands. Using a time-lapse imaging system, we found that PDAC microtumours actively stretched to catch dead cell debris via filo/lamellipoedia and suction, suggesting that they have a sophisticated survival strategy (analogous to that of starving animals), which implies a context for the development of possible therapies for PDACs. The simple tumour imaging system visualises a potential of PDAC cells, in which the aggressive tumour dynamics reminds us of the need to review traditional PDAC pathogenesis.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮武 由甲子

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