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Enzymatically synthesized megalo-type isomaltosaccharides enhance the barrier function of the tight junction in the intestinal epithelium
| Title: | Enzymatically synthesized megalo-type isomaltosaccharides enhance the barrier function of the tight junction in the intestinal epithelium |
| Authors: | Hara, Hiroshi Browse this author →KAKEN DB | | Kume, Shunsuke Browse this author | | Iizuka, Takahisa Browse this author | | Fujimoto, Yoshinori Browse this author | | Kimura, Atsuo Browse this author |
| Keywords: | Isomaltosaccharide | | Tight junction | | Intestinal barrier | | Caveolae |
| Issue Date: | 27-Nov-2017 |
| Publisher: | Taylor & Francis |
| Journal Title: | Bioscience, Biotechnology, and Biochemistry |
| Volume: | 82 |
| Issue: | 4 |
| Start Page: | 629 |
| End Page: | 635 |
| Publisher DOI: | 10.1080/09168451.2017.1398065 |
| PMID: | 29173029 |
| Abstract: | Megalo-type isomaltosaccharides are an enzymatically synthesized foodstuff produced by transglucosylation from maltodextrin, and they contain a mid-chain length polymer of D-glucose with -1,6-glycoside linkages. The injection of a solution of megalo-type isomaltosaccharides (1-4%(w/v), average DP=12.6), but not oligo-type isomaltosaccharides (average DP=3.3), into the intestinal lumen dose-dependently reduced the transport rates of tight junction permeable markers in a ligated loop of the anesthetized rat jejunum. Application of the megalosaccharide also suppressed the transport of tight junction markers and enhanced transepithelial electrical resistance (TEER) in Caco-2 cell monolayers. Cholesterol sequestration by methyl--cyclodextrin in the Caco-2 monolayers abolished the effect of megalosaccharide. Treatment with anti-caveolin-1 and a caveolae inhibitor, but not clathrin-dependent endocytosis and macropinocytosis inhibitors, suppressed the increase in TEER. These results indicate that isomaltosaccharides promote the barrier function of tight junctions in the intestinal epithelium in a chain-length dependent manner and that caveolae play a role in the effect. |
| Rights: | This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry 82(4) on 2018, available online: https://www.tandfonline.com/doi/full/10.1080/09168451.2017.1398065 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/72039 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 原 博
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