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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Ovarian mast cells migrate toward ovary- fimbria connection in neonatal MRL/MpJ mice
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| Title: | Ovarian mast cells migrate toward ovary- fimbria connection in neonatal MRL/MpJ mice |
| Authors: | Nakamura, Teppei Browse this author | | Chihara, Masataka Browse this author | | Ichii, Osamu Browse this author →KAKEN DB | | Otsuka-Kanazawa, Saori Browse this author →KAKEN DB | | Nagasaki, Ken-ichi Browse this author | | Elewa, Yaser Hosny Ali Browse this author →ORCID | | Tatsumi, Osamu Browse this author | | Kon, Yasuhiro Browse this author →KAKEN DB |
| Issue Date: | 23-Apr-2018 |
| Publisher: | PLOS |
| Journal Title: | PLoS ONE |
| Volume: | 13 |
| Issue: | 4 |
| Start Page: | e0196364 |
| Publisher DOI: | 10.1371/journal.pone.0196364 |
| Abstract: | MRL/MpJ mice have abundant ovarian mast cells (MCs) as compared with other strains at postnatal day 0 (P0); however, they sharply decrease after birth. These ovarian MCs, particularly beneath the ovarian surface epithelium (SE), which express mucosal MC (MMC) marker, might participate in early follicular development. This study investigated the changes in spatiotemporal distribution of MCs in the perinatal MRL/MpJ mouse ovaries. At P0 to P7, the MCs were densely localized to the ovary, especially their caudomedial region around the ovary-fimbria connection. The neonatal ovarian MCs showed intermediate characteristics of MMC and connective tissue MC (CTMC), and the latter phenotype became evident with aging. However, the expression ratio of the MMC to CTMC marker increased from P0 to P4 in the MRL/MpJ mouse ovary. Similarly, the ratio of MCs facing SE to total MC number increased with aging, although the number of ovarian MCs decreased, indicating the relative increase in MMC phenotypes in the early neonatal ovary. Neither proliferating nor apoptotic MCs were found in the MRL/MpJ mouse ovaries. The parenchymal cells surrounding MCs at ovary-fimbria connection showed similar molecular expression patterns (E-cadherin(+)/Foxl2(-)/Gata4(+)) as that of the ovarian surface epithelial cells. At P2, around the ovary-fimbria connection, c-kit - immature oocytes formed clusters called nests, and some MCs localized adjacent to c-kit oocytes within the nests. These results indicated that in postnatal MRL/MpJ mice, ovarian MCs changed their distribution by migrating toward the parenchymal cells composing ovary-fimbria connection, which possessed similar characteristics to the ovarian surface epithelium. Thus, we elucidated the spatiotemporal alterations of the ovarian MCs in MRL/MpJ mice, and suggested their importance during the early follicular development by migrating toward the ovary-fimbria connection. MRL/MpJ mice would be useful to elucidate the relationship between neonatal immunity and reproductive systems. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/72160 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 昆 泰寛
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