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Enhanced expression of cysteine-rich antimicrobial peptide snakin-1 in Escherichia coli using an aggregation-prone protein coexpression system

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Title: Enhanced expression of cysteine-rich antimicrobial peptide snakin-1 in Escherichia coli using an aggregation-prone protein coexpression system
Authors: Kuddus, Md. Ruhul Browse this author
Yamano, Megumi Browse this author
Rumi, Farhana Browse this author
Kikukawa, Takashi Browse this author →KAKEN DB
Demura, Makoto Browse this author →KAKEN DB
Aizawa, Tomoyasu Browse this author →KAKEN DB
Keywords: recombinant expression
antimicrobial peptide
snakin-1
inclusion body
Escherichia coli
Issue Date: Dec-2017
Publisher: John Wiley & Sons
Journal Title: Biotechnology progress
Volume: 33
Issue: 6
Start Page: 1520
End Page: 1528
Publisher DOI: 10.1002/btpr.2508
PMID: 28556600
Abstract: Snakin-1 (SN-1) is a cysteine-rich plant antimicrobial peptide and the first purified member of the snakin family. SN-1 shows potent activity against a wide range of microorganisms, and thus has great biotechnological potential as an antimicrobial agent. Here, we produced recombinant SN-1 in Escherichia coli by a previously developed coexpression method using an aggregation-prone partner protein. Our goal was to increase the productivity of SN-1 via the enhanced formation of insoluble inclusion bodies in E. coli cells. The yield of SN-1 by the coexpression method was better than that by direct expression in E. coli cells. After refolding and purification, we obtained several milligrams of functionally active SN-1, the identity of which was verified by MALDI-TOF MS and NMR studies. The purified recombinant SN-1 showed effective antimicrobial activity against test organisms. Our studies indicate that the coexpression method using an aggregation-prone partner protein can serve as a suitable expression system for the efficient production of functionally active SN-1. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1520–1528, 2017
Rights: This is the peer reviewed version of the following article: Kuddus, Md. R., Yamano, M., Rumi, F., Kikukawa, T., Demura, M. and Aizawa, T. (2017), Enhanced expression of cysteine-rich antimicrobial peptide snakin-1 in Escherichia coli using an aggregation-prone protein coexpression system. Biotechnol Progress, 33: 1520–1528. doi:10.1002/btpr.2508, which has been published in final form at http://dx.doi.org/10.1002/btpr.2508. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
URI: http://hdl.handle.net/2115/72253
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 相沢 智康

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