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Immunolocalization of DMP1 and sclerostin in the epiphyseal trabecule and diaphyseal cortical bone of osteoprotegerin deficient mice

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Title: Immunolocalization of DMP1 and sclerostin in the epiphyseal trabecule and diaphyseal cortical bone of osteoprotegerin deficient mice
Authors: Masuki, H. Browse this author
Li, Minqi Browse this author →KAKEN DB
Hasegawa, T. Browse this author →KAKEN DB
Suzuki, R. Browse this author →KAKEN DB
Guo, Y. Browse this author
Liu, Z. Browse this author
Oda, K. Browse this author →KAKEN DB
Yamamoto, T. Browse this author →KAKEN DB
Kawanami, M. Browse this author →KAKEN DB
Amizuka, N. Browse this author →KAKEN DB
Issue Date: 10-Nov-2010
Publisher: Biomedical Research Press
Journal Title: Biomedical Research
Volume: 31
Issue: 5
Start Page: 307
End Page: 318
Publisher DOI: 10.2220/biomedres.31.307
Abstract: In order to define the osteocytic function in accelerated bone remodeling, we examined the distribution of the osteocytic lacunar-canalicular system (OLCS) and osteocyte-secreting molecules-dentin matrix protein (DMP) 1 and sclerostin-in the epiphyses and cortical bones of osteoprotegerin deficient (OPG-/-) mice. Silver impregnation visualized a well-arranged OLCS in the wild-type epiphyses and cortical bone, whereas OPG-/- mice had an irregular OLCS in the epiphyses, but well-arranged canaliculi in the cortical bone. DMP1-positive osteocytes were evenly distributed throughout the wild-type epiphyses and cortical bone, as well as the OPG-/- cortical bone. However, OPG-/- epiphyses revealed weak DMP1-immunoreactivity. Thus, osteocytes appear to synthesize more DMP1 as the OLCS becomes regular. In contrast, sclerostin-immunoreactivity was significantly diminished in the OPG-/- epiphyses and cortical bone. In OPG-/- epiphyses and cortical bone, triple staining demonstrated few sclerostin-positive osteocytes in the periphery of a thick cell layer of alkaline phosphatase-positive osteoblasts and many tartrate resistant acid phosphatasepositive osteoclasts. Summarizing, the regular distribution of OLCS may affect DMP1 synthesis, while the cellular activities of osteoclasts and osteoblasts rather than the regularity of OLCS may ultimately influence sclerostin synthesis.
Type: article
URI: http://hdl.handle.net/2115/72271
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷川 智香

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