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Hertwig's Epithelial Root Sheath Fate during Initial Cellular Cementogenesis in Rat Molars

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Title: Hertwig's Epithelial Root Sheath Fate during Initial Cellular Cementogenesis in Rat Molars
Authors: Yamamoto, T. Browse this author →KAKEN DB
Yamada, T. Browse this author →KAKEN DB
Yamamoto, T. Browse this author
Hasegawa, T. Browse this author →KAKEN DB
Hongo, H. Browse this author
Oda, K. Browse this author →KAKEN DB
Amizuka, N. Browse this author →KAKEN DB
Keywords: Hertwig’s epithelial root sheath
epithelial-mesenchymal transition
cellular cementum
rat molars
Issue Date: 29-Jun-2015
Publisher: Japan Society of Histochemistry and Cytochemistry
Journal Title: Acta Histochemica et Cytochemica
Volume: 48
Issue: 3
Start Page: 95
End Page: 101
Publisher DOI: 10.1267/ahc.15006
Abstract: To elucidate the fate of the epithelial root sheath during initial cellular cementogenesis, we examined developing maxillary first molars of rats by immunohistochemistry for keratin, vimentin, and tissue non-specific alkaline phosphatase (TNALP) and by TdT-mediated dUTP nick end labeling (TUNEL). The advancing root end was divided into three sections, which follow three distinct stages of initial cellular cementogenesis: section 1, where the epithelial sheath is intact; section 2, where the epithelial sheath becomes fragmented; and section 3, where initial cellular cementogenesis begins. After fragmentation of the epithelial sheath, many keratin-positive epithelial sheath cells were embedded in the rapidly growing cellular cementum. A few unembedded epithelial cells located on the cementum surface. Dental follicle cells, precementoblasts, and cementoblasts showed immunoreactivity for vimentin and TNALP. In all three sections, there were virtually no cells possessing double immunoreactivity for vimentin-keratin or TNALP-keratin and only embedded epithelial cells showed TUNEL reactivity. Taken together, these findings suggest that: (1) epithelial sheath cells divide into two groups; one group is embedded in the cementum and thereafter dies by apoptosis, and the other survives on the cementum surface as epithelial cell rests of Malassez; and (2) epithelial sheath cells do not undergo epithelial-mesenchymal transition during initial cellular cementogenesis.
Rights: 著作権は日本組織細胞化学会が所有
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷川 智香

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