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Immunolocalization of osteocyte-derived molecules during bone fracture healing of mouse ribs

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/72276

Title: Immunolocalization of osteocyte-derived molecules during bone fracture healing of mouse ribs
Authors: Liu, Z. Browse this author
Yamamoto, T. Browse this author
Hasegawa, T. Browse this author →KAKEN DB
Hongo, H. Browse this author
Tsuboi, K. Browse this author
Tsuchiya, E. Browse this author
Haraguchi, M. Browse this author
Abe, M. Browse this author
Freitas, PHL. Browse this author
Kudo, A. Browse this author
Oda, K. Browse this author
Li, M. Browse this author
Amizuka, N. Browse this author →KAKEN DB
Issue Date: 1-Apr-2016
Publisher: Biomedical Research Press
Journal Title: Biomedical Research
Volume: 37
Issue: 2
Start Page: 141
End Page: 151
Publisher DOI: 10.2220/biomedres.37.141
PMID: 27108883
Abstract: We employed a well-standardized murine rib fracture model to assess the distribution, in the cortical bone, of three important osteocyte-derived molecules—dentine matrix protein 1 (DMP1), sclerostin and fibroblast growth factor 23 (FGF 23). Two days after the fracture, the periosteum thickened, and up to the seventh day post-fracture, the cortical surfaces were promoting neoformation of two tissue types depending on the distance from the fracture site: chondrogenesis was taking place near the fracture, and osteogenesis distant from it. The cortical bones supporting chondrogenesis featured several empty lacunae, while in the ones underlying newly-formed woven bone, empty lacunae were hardly seen. DMP1-immunopositive osteocytic lacunae and canaliculi were seen both close and away from the fracture. In contrast, the region close to the fracture had only few sclerostin- and FGF23-immunoreactive osteocytes, whereas the distant region revealed many osteocytes immunopositive for these markers. Mature cortical bone encompassing the native cortical bone was observed at two-, three- and four-weeks post-fracture, and the distribution of DMP1, sclerostin and FGF23 appeared to have returned to normal. In summary, early stages of fracture healing seem to be important for triggering chondrogenesis and osteogenesis that may be regulated by osteocytes via their secretory molecules.
Type: article
URI: http://hdl.handle.net/2115/72276
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷川 智香

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