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Histological assessment for femora of ovariectomized obesity (db/db) mice carrying mutated leptin receptor

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Title: Histological assessment for femora of ovariectomized obesity (db/db) mice carrying mutated leptin receptor
Authors: Tanaka, Y. Browse this author
Hasegawa, T. Browse this author →KAKEN DB
Yamada, T. Browse this author →KAKEN DB
Yamamoto, T. Browse this author
Sasaki, M. Browse this author
Hongo, H. Browse this author
Tsuboi, K. Browse this author
Haraguchi, M. Browse this author
Freitas, de PHL. Browse this author
Li, M. Browse this author →KAKEN DB
Oda, K. Browse this author →KAKEN DB
Totsuka, Y. Browse this author →KAKEN DB
Tei, Kanchu Browse this author →KAKEN DB
Amizuka, N. Browse this author →KAKEN DB
Keywords: db/db mice
Issue Date: Dec-2016
Publisher: University of Murcia
Journal Title: Histology and Histopathology
Volume: 31
Issue: 12
Start Page: 1315
End Page: 1326
Publisher DOI: 10.14670/HH-11-758
PMID: 26984199
Abstract: In order to provide a clue to understand the interplay between leptin and estrogen, we have examined femoral metaphyses of ovariectomized db/db mice carrying a mutated leptin receptor. We performed ovariectomy (OVX) or sham-operation (sham) on 12-week old female wild-type and db/db mice, and then, after 8 weeks, divided the animals into four groups: wild-type sham, wild-type OVX, db/db sham and db/db OVX. Samples from all groups were prepared for histochemical and ultrastructural examinations. As a result, db/db sham mice showed a reduced number and thickness of metaphyseal trabeculae and excessive adipose tissue when compared to wild-type sham mice. The wild-type OVX group exhibited markedly diminished trabecular number, as well as lower populations of osteoblasts and osteoclasts in comparison to wild-type sham group. On the other hand, trabecular numbers were similar for the two db/db groups, suggesting that the effect of the ovariectomy, i.e., estrogen deficiency may be lessened in this animal model. Leptin receptor was mainly found in osteoblasts and in bone marrow stromal cells including adipocytes. In addition, the expression of estrogen receptor did not seem to change after OVX in wild-type mice and in db/db mice. Both db/db sham and OVX mice featured many adipocytes close to the metaphyseal chondro-osseous junction, while osteoblasts accumulated glycogen granules and lipid droplets. Therefore, it seems likely that the disruption of leptin signaling in db/db mice shifts the cell differentiation cascade towards the adipocyte lineage, resulting in an osteoporotic bone independently of estrogen deficiency.
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷川 智香

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