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Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/72290

Title: Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone
Authors: Sakurai, A. Browse this author
Hasegawa, T. Browse this author →KAKEN DB
Kudo, A. Browse this author
Shen, Z. Browse this author
Nagai, T. Browse this author
Abe, M. Browse this author
Yoshida, T. Browse this author
Hongo, H. Browse this author
Yamamoto, T. Browse this author
Yamamoto, T. Browse this author →KAKEN DB
Oda, K. Browse this author →KAKEN DB
Freitas, de PHL. Browse this author
Li, M. Browse this author →KAKEN DB
Sano, H. Browse this author →KAKEN DB
Amizuka, N. Browse this author →KAKEN DB
Issue Date: 1-Aug-2017
Publisher: Biomedical Research Press
Journal Title: Biomedical Research
Volume: 38
Issue: 4
Start Page: 257
End Page: 267
Publisher DOI: 10.2220/biomedres.38.257
PMID: 28794403
Abstract: To assess the chronological participation of sclerostin and FGF23 in bone metabolism, this study tracked the immunolocalization of sclerostin and FGF23 in the metaphyses of murine long bones from embryonic day 18 (E18) through 1 day after birth, 1 week, 2 weeks, 4 weeks, 8 weeks, and 20 weeks of age. We have selected two regions in the metaphyseal trabeculae for assessing sclerostin and FGF23 localization: close to the chondro-osseous junction, i.e., bone modeling site even in the adult animals, and the trabecular region distant from the growth plate, where bone remodeling takes place. As a consequence, sclerostin-immunopositive osteocytes could not be observed in both close and distant trabecular regions early at the embryonic and young adult stages. However, osteocytes gradually started to express sclerostin in the distant region earlier than in the close region of the trabeculae. Immunoreactivity for FGF23 was observed mainly in osteoblasts in the early stages, but detectable in osteocytes in the later stages of growth in trabecular and cortical bones. Fgf23 was weakly expressed in the embryonic and neonatal stages, while the receptors, Fgfr1c and αKlotho were strongly expressed in femora. At the adult stages, Fgf23 expression became more intense while Fgfr1c and aKlotho were weakly expressed. These findings suggest that sclerostin is secreted by osteocytes in mature bone undergoing remodeling while FGF23 is synthesized by osteoblasts and osteocytes depending on the developmental/growth stage. In addition, it appears that FGF23 acts in an autocrine and paracrine fashion in fetal and neonatal bones.
Type: article
URI: http://hdl.handle.net/2115/72290
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷川 智香

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