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Morphological assessment of bone mineralization in tibial metaphyses of ascorbic acid-deficient ODS rats

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Title: Morphological assessment of bone mineralization in tibial metaphyses of ascorbic acid-deficient ODS rats
Authors: Hasegawa, T. Browse this author →KAKEN DB
Li, M. Browse this author →KAKEN DB
Hara, K. Browse this author
Sasaki, M. Browse this author
Tabata, C. Browse this author
Freitas, PHL. Browse this author
Hongo, H. Browse this author
Suzuki, R. Browse this author →KAKEN DB
Kobayashi, M. Browse this author
Inoue, K. Browse this author →KAKEN DB
Yamamoto, T. Browse this author →KAKEN DB
Oohata, N. Browse this author
Oda, K. Browse this author →KAKEN DB
Akiyama, Y. Browse this author
Amizuka, N. Browse this author →KAKEN DB
Issue Date: 30-Aug-2011
Publisher: Biomedical Research Press
Journal Title: Biomedical Research
Volume: 32
Issue: 4
Start Page: 259
End Page: 269
Publisher DOI: 10.2220/biomedres.32.259
Abstract: Osteogenic disorder shionogi (ODS) rats carry a hereditary defect in ascorbic acid synthesis, mimicking human scurvy when fed with an ascorbic acid-deficient (aa-def) diet. As aa-def ODS rats were shown to feature disordered bone formation, we have examined the bone mineralization in this rat model. A fibrous tissue layer surrounding the trabeculae of tibial metaphyses was found in aa-def ODS rats, and this layer showed intense alkaline phosphatase activity and proliferating cell nuclear antigen-immunopositivity. Many osteoblasts detached from the bone surfaces and were characterized by round-shaped rough endoplasmic reticulum (rER), suggesting accumulation of malformed collagen inside the rER. Accordingly, fine, fragile fibrillar collagenous structures without evident striation were found in aa-def bones, which may result from misassembling of the triple helices of collagenous α-chains. Despite a marked reduction in bone formation, ascorbic acid deprivation seemed to have no effect on mineralization: while reduced in number, normal matrix vesicles and mineralized nodules could be seen in aa-def bones. Fine needle-like mineral crystals extended from these mineralized nodules, and were apparently bound to collagenous fibrillar structures. In summary, collagen mineralization seems unaffected by ascorbic acid deficiency in spite of the fine, fragile collagenous fibrils identified in the bones of our animal model.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷川 智香

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