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Sclerostin is differently immunolocalized in metaphyseal trabecules and cortical bones of mouse tibiae

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/72293

Title: Sclerostin is differently immunolocalized in metaphyseal trabecules and cortical bones of mouse tibiae
Authors: Hasegawa, T. Browse this author →KAKEN DB
Amizuka, N. Browse this author →KAKEN DB
Yamada, T. Browse this author →KAKEN DB
Liu, Z. Browse this author
Miyamoto, Y. Browse this author
Yamamoto, T. Browse this author
Sasaki, M. Browse this author
Hongo, H. Browse this author
Suzuki, R. Browse this author →KAKEN DB
Freitas, PHL. Browse this author
Yamamoto, T. Browse this author →KAKEN DB
Oda, K. Browse this author →KAKEN DB
Li, M. Browse this author →KAKEN DB
Issue Date: 19-Jun-2013
Publisher: Biomedical Research Press
Journal Title: Biomedical Research
Volume: 34
Issue: 3
Start Page: 153
End Page: 159
Publisher DOI: 10.2220/biomedres.34.153
Abstract: Sclerostin, an osteocyte-derived molecule, has been reported to serve as a negative regulator of osteoblastic activity as well as bone remodeling. However, there is no report that verified the regional difference for sclerostin synthesis, and in this study we have investigated immunolocalization of sclerostin by comparing dentin matrix protein (DMP) 1, an osteocyte-derived factor broadly expressed in tibial metaphyses and cortical bone. In metaphyseal primary trabecules, a site of bone modeling, strong DMP1-reactivity was observed in osteocytic lacunar-canalicular system (OLCS), while faint staining for sclerostin was visible only in a few osteocytes. In secondary trabecules, in which bone remodeling begins, some osteocytes showed intense sclerostin-immunopositivity, though there were many DMP1-positive osteocytes. In cortical bone, there were more osteocytes reactive for sclerostin, when compared with those in the secondary trabecules. Silver impregnation verified that immature, primary trabecules contained randomly-oriented OLCS, while mature, cortical bone showed geometrically well-arrangement of OLCS. Taken together, though DMP1 is broadly synthesized in bone, sclerostin appears to be abundantly synthesized in regular OLCS of cortical bone, but less produced in irregular OLCS as seen in primary trabecules, indicating the regional difference for sclerostin synthesis.
Type: article
URI: http://hdl.handle.net/2115/72293
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷川 智香

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