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Histochemical aspects of the vascular invasion at the erosion zone of the epiphyseal cartilage in MMP-9-deficient mice

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Title: Histochemical aspects of the vascular invasion at the erosion zone of the epiphyseal cartilage in MMP-9-deficient mice
Authors: Kojima, T. Browse this author
Hasegawa, T. Browse this author →KAKEN DB
Freitas, PHL. Browse this author
Yamamoto, T. Browse this author
Sasaki, M. Browse this author
Horiuchi, K. Browse this author
Hongo, H. Browse this author
Yamada, T. Browse this author →KAKEN DB
Sakagami, N. Browse this author
Saito, N. Browse this author
Yoshizawa, M. Browse this author
Kobayashi, T. Browse this author
Maeda, T. Browse this author
Saito, C. Browse this author
Amizuka, N. Browse this author →KAKEN DB
Issue Date: 19-Jun-2013
Publisher: Biomedical Research Press
Journal Title: Biomedical Research
Volume: 34
Issue: 3
Start Page: 119
End Page: 128
Publisher DOI: 10.2220/biomedres.34.119
Abstract: We have histologically examined vascular invasion and calcification of the hypertrophic zone during endochondral ossification in matrix metalloproteinase (MMP)-9 deficient (MMP-9-/-) mice and in their littermates at 3 days, 3 weeks and 6 weeks after birth. Capillaries and osteoclasts at the chondro-osseous junction showed an intense MMP-9 immunopositivity, suggesting that they recognize chemical properties of cartilaginous matrices, and then release MMP-9 for cartilage degradation. CD31-positive capillaries and tartrate-resistant acid phosphatase-reactive osteoclasts could be found in the close proximity in the region of chondro-osseous junction in MMP-9-/- mice, while in wild-type mice, vascular invasion preceded osteoclastic migration into the epiphyseal cartilage. Although MMP-9-/- mice revealed larger hypertrophic zones, the index of calcified area was significantly smaller in MMP-9-/- mice. Interestingly, the lower layer of the MMP-9-/- hypertrophic zone showed intense MMP-13 staining, which could not be observed in wild-type mice. This indicates that MMP-13 may compensate for MMP-9 deficiency at that specific region, but not to a point at which the deficiency could be completely rescued. In conclusion, it seems that MMP-9 is the optimal enzyme for cartilage degradation during endochondral ossification by controlling vascular invasion and subsequent osteoclastic migration.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷川 智香

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