| Title: | Histochemical aspects of the vascular invasion at the erosion zone of the epiphyseal cartilage in MMP-9-deficient mice |
| Authors: | Kojima, T. Browse this author |
| Hasegawa, T. Browse this author →KAKEN DB |
| Freitas, PHL. Browse this author |
| Yamamoto, T. Browse this author |
| Sasaki, M. Browse this author |
| Horiuchi, K. Browse this author |
| Hongo, H. Browse this author |
| Yamada, T. Browse this author →KAKEN DB |
| Sakagami, N. Browse this author |
| Saito, N. Browse this author |
| Yoshizawa, M. Browse this author |
| Kobayashi, T. Browse this author |
| Maeda, T. Browse this author |
| Saito, C. Browse this author |
| Amizuka, N. Browse this author →KAKEN DB |
| Issue Date: | 19-Jun-2013 |
| Publisher: | Biomedical Research Press |
| Journal Title: | Biomedical Research |
| Volume: | 34 |
| Issue: | 3 |
| Start Page: | 119 |
| End Page: | 128 |
| Publisher DOI: | 10.2220/biomedres.34.119 |
| Abstract: | We have histologically examined vascular invasion and calcification of the hypertrophic zone during endochondral ossification in matrix metalloproteinase (MMP)-9 deficient (MMP-9-/-) mice and in their littermates at 3 days, 3 weeks and 6 weeks after birth. Capillaries and osteoclasts at the chondro-osseous junction showed an intense MMP-9 immunopositivity, suggesting that they recognize chemical properties of cartilaginous matrices, and then release MMP-9 for cartilage degradation. CD31-positive capillaries and tartrate-resistant acid phosphatase-reactive osteoclasts could be found in the close proximity in the region of chondro-osseous junction in MMP-9-/- mice, while in wild-type mice, vascular invasion preceded osteoclastic migration into the epiphyseal cartilage. Although MMP-9-/- mice revealed larger hypertrophic zones, the index of calcified area was significantly smaller in MMP-9-/- mice. Interestingly, the lower layer of the MMP-9-/- hypertrophic zone showed intense MMP-13 staining, which could not be observed in wild-type mice. This indicates that MMP-13 may compensate for MMP-9 deficiency at that specific region, but not to a point at which the deficiency could be completely rescued. In conclusion, it seems that MMP-9 is the optimal enzyme for cartilage degradation during endochondral ossification by controlling vascular invasion and subsequent osteoclastic migration. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/72294 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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