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Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility

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Title: Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility
Authors: Kanaoka, Yoshitaka Browse this author
Kuniyoshi, Daichi Browse this author
Inada, Eri Browse this author
Koide, Yohei Browse this author
Okamoto, Yoshihiro Browse this author
Yasui, Hideshi Browse this author
Kishima, Yuji Browse this author →KAKEN DB
Keywords: Anther culture
Callus
Hybrid sterility
Mapping
Microspore
Oryza sativa
O. glaberrima
Rice
Issue Date: 17-Nov-2018
Publisher: BioMed Central
Journal Title: Plant methods
Volume: 14
Start Page: 102
Publisher DOI: 10.1186/s13007-018-0370-z
Abstract: BackgroundTo investigate plant hybrid sterility, we studied interspecific hybrids of two cultivated rice species, Asian rice (Oryza sativa) and African rice (O. glaberrima). Male gametes of these hybrids display complete sterility owing to a dozen of hybrid sterility loci, termed HS loci, but this complicated genetic system remains poorly understood.ResultsMicrospores from these interspecific hybrids form sterile pollen but are viable at the immature stage. Application of the anther culture (AC) method caused these immature microspores to induce callus. The segregation distortion of 11 among 13 known HS loci was assessed in the callus population. Using many individual calli, fine mapping of the HS loci was attempted based on heterozygotes produced from chromosome segment substitution lines (CSSLs). Transmission ratio distortion (TRD) from microspores was detected at 6 of 11 HS loci in the callus population. The fine mapping of S-1 and S-19 loci using CSSLs revealed precise distances of markers from the positions of HS loci exhibiting excessive TRD.ConclusionsWe demonstrated that AC to generate callus populations derived from immature microspores is a useful methodology for genetic study. The callus population facilitated detection of TRD at multiple HS loci and dramatically shortened the process for mapping hybrid sterility genes.
Rights: © The Author(s) 2018
https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/72300
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 貴島 祐治

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