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Effects of non-equilibrium angle fluctuation on F-1-ATPase kinetics induced by temperature increase

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Title: Effects of non-equilibrium angle fluctuation on F-1-ATPase kinetics induced by temperature increase
Authors: Tamiya, Yuji Browse this author
Watanabe, Rikiya Browse this author →KAKEN DB
Noji, Hiroyuki Browse this author →KAKEN DB
Li, Chun-Biu Browse this author
Komatsuzaki, Tamiki Browse this author →KAKEN DB
Issue Date: 21-Jan-2018
Publisher: Royal Society of Chemistry
Journal Title: Physical chemistry chemical physics
Volume: 20
Issue: 3
Start Page: 1872
End Page: 1880
Publisher DOI: 10.1039/c7cp06256g
PMID: 29292807
Abstract: F-1-ATPase (F-1) is an efficient rotary protein motor, whose reactivity is modulated by the rotary angle to utilize thermal fluctuation. In order to elucidate how its kinetics are affected by the change in the fluctuation, we have extended the reaction-diffusion formalism [R. Watanabe et al., Biophys. J., 2013, 105, 2385] applicable to a wider range of temperatures based on experimental data analysis of F1 derived from thermophilic Bacillus under high ATP concentration conditions. Our simulation shows that the rotary angle distribution manifests a stronger non-equilibrium feature as the temperature increases, because ATP hydrolysis and Pi release are more accelerated compared with the timescale of rotary angle relaxation. This effect causes the rate coefficient obtained from dwell time fitting to deviate from the Arrhenius relation in Pi release, which has been assumed in the previous activation thermodynamic quantities estimation using linear Arrhenius fitting. Larger negative correlation is also found between hydrolysis and Pi release waiting time in a catalytic dwell with the increase in temperature. This loss of independence between the two successive reactions at the catalytic dwell sheds doubt on the conventional dwell time fitting to obtain rate coefficients with a double exponential function at temperatures higher than 65 degrees C, which is close to the physiological temperature of the thermophilic Bacillus.
Type: article (author version)
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小松崎 民樹

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