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Functional modulation of caecal fermentation and microbiota in rat by feeding bean husk as a dietary fibre supplement

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Title: Functional modulation of caecal fermentation and microbiota in rat by feeding bean husk as a dietary fibre supplement
Authors: Myint, H. Browse this author
Kishi, H. Browse this author
Iwahashi, Y. Browse this author
Saburi, W. Browse this author
Koike, S. Browse this author
Kobayashi, Y. Browse this author →KAKEN DB
Keywords: bean husk
fermentation metabolites
gut health
microbiota
Issue Date: 22-Jan-2019
Publisher: Wageningen Academic Publishers
Journal Title: Beneficial microbes
Volume: 9
Issue: 6
Start Page: 963
End Page: 974
Publisher DOI: 10.3920/BM2017.0174
PMID: 30208728
Abstract: A feeding study using rats was conducted to evaluate the utility of lablab bean husk and soya bean husk as sources of potential prebiotic fibre. Twenty 5-week-old Sprague Dawley rats were divided into 4 groups and fed one of the following diets for 3 weeks: purified diet (AIN93 G) containing 5% cellulose (CEL), or the same diet in which cellulose was replaced by corn starch (STA), lablab bean husk (LBH), or soya bean husk (SBH). Rats were sacrificed at 8 weeks of age and caecal digesta were collected. Feed intake, body weight, anatomical parameters, and caecal ammonia level did not differ significantly among diets. Rats on LBH and SBH showed higher concentrations of caecal short-chain fatty acid and lactate than those on CEL. Rats on CEL, SBH, and LBH exhibited lower caecal indole and skatole levels. LBH yielded increased caecal abundance of Akkermansia muciniphila and Oscillibacter relatives, as demonstrated by either qPCR, MiSeq, or clone library analysis. SBH favoured the growth of lactobacilli as assessed by both qPCR and MiSeq, and favoured the growth of bifidobacteria as assessed by MiSeq. In comparison with STA, LBH and SBH yielded lower caecal abundance of bacteria related to Dorea massiliensis, as demonstrated by qPCR, MiSeq, and clone library analysis. Both types of bean husk were found to contain oligosaccharides that might selectively stimulate the growth of beneficial bacteria. Based on these results, the two species of bean husk tested are considered potentially functional for promoting the gut health of monogastric animals.
Rights: © 2018 Wageningen Academic Publishers
http://creativecommons.org/licenses/by-nc-sa/4.0/
Type: article
URI: http://hdl.handle.net/2115/72349
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小林 泰男

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