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Hokkaido University Collection of Scholarly and Academic Papers >
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Adiponectin suppression of late inflammatory mediator, HMGB1-induced cytokine expression in RAW264 macrophage cells
| Title: | Adiponectin suppression of late inflammatory mediator, HMGB1-induced cytokine expression in RAW264 macrophage cells |
| Authors: | Elfeky, Mohamed Browse this author | | Yoneshiro, Takeshi Browse this author | | Okamatsu-Ogura, Yuko Browse this author →KAKEN DB | | Kimura, Kazuhiro Browse this author →KAKEN DB |
| Keywords: | adiponectin | | high-mobility group protein B1 | | inflammation | | macrophages | | toll-like receptor 4 |
| Issue Date: | Feb-2018 |
| Publisher: | Oxford University Press |
| Journal Title: | Journal of biochemistry |
| Volume: | 163 |
| Issue: | 2 |
| Start Page: | 143 |
| End Page: | 153 |
| Publisher DOI: | 10.1093/jb/mvx069 |
| PMID: | 29048484 |
| Abstract: | High-mobility group protein B1 (HMGB1) is a late inflammatory mediator released from inflammatory cells when stimulated, resulting in exaggerating septic symptoms. We recently demonstrated that full-length adiponectin, a potent anti-inflammatory adipokine, inhibits lipopolysaccharide-induced HMGB1 release. However, the effects of adiponectin on HMGB1-induced exaggerating signals currently remain unknown. This study aimed to investigate the effects of adiponectin on the pro-inflammatory function of HMGB1 in RAW264 macrophage cells. The treatment of RAW264 cells with HMGB1 significantly up-regulated the mRNA expression of tumour necrosis factor-alpha, interleukin-1 beta and C-X-C motif chemokine 10. HMGB1-induced cytokine expression was markedly suppressed by a toll-like receptor 4 (TLR4) antagonist and slightly suppressed by an antagonist of the receptor for advanced glycation end products. A prior treatment with full-length or globular adiponectin dose-dependently suppressed all types of HMGB1-induced cytokine expression, and this suppression was abolished by compound C, an AMPK inhibitor, but not by the haem oxygenase (HO)-1 inhibitor, zinc protoporphyrin IX. Both forms of adiponectin also reduced the mRNA expression of TLR4. These results suggest that full-length and globular adiponectin suppress HMGB1-induced cytokine expression through an AMPK-mediated HO-1-independent pathway. |
| Rights: | This is a pre-copyedited, author-produced version of an article accepted for publication in The journal of biochemistry following peer review. The version of record Volume 163, Issue 2, Pages 143-153, 2018 is available online at: https://doi.org/10.1093/jb/mvx069. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/72449 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 和弘
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