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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Urinary Exosome-Derived microRNAs Reflecting the Changes in Renal Function in Cats
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| Title: | Urinary Exosome-Derived microRNAs Reflecting the Changes in Renal Function in Cats |
| Authors: | Ichii, Osamu Browse this author →KAKEN DB | | Ohta, Hiroshi Browse this author →KAKEN DB | | Horino, Taro Browse this author | | Nakamura, Teppei Browse this author | | Hosotani, Marina Browse this author | | Mizoguchi, Tatsuya Browse this author | | Morishita, Keitaro Browse this author →KAKEN DB | | Nakamura, Kensuke Browse this author | | Sasaki, Noboru Browse this author | | Takiguchi, Mitsuyoshi Browse this author →KAKEN DB | | Sato, Ryo Browse this author | | Oyamada, Kazuhisa Browse this author | | Elewa, Yaser Hosny Ali Browse this author →ORCID | | Kon, Yasuhiro Browse this author →KAKEN DB |
| Keywords: | urinary exosome-derived miRNA | | cats | | kidney disease | | biomarker | | next-generation sequencing |
| Issue Date: | 20-Nov-2018 |
| Publisher: | Frontiers Media |
| Journal Title: | Frontiers in veterinary science |
| Volume: | 5 |
| Start Page: | 289 |
| Publisher DOI: | 10.3389/fvets.2018.00289 |
| Abstract: | Increased incidence of kidney disease (KD) is a common concern in human and companion animals. Cats, in particular, are highly susceptible to KD. Novel KD biomarkers would help to address these problems. Therefore, we are focusing on microRNA, a highly conserved nucleic acid, as a KD biomarker for various animals. We previously reported that altered levels of urinary exosome (UExo)-derived microRNAs indicate renal pathologies in dogs. This study comprehensively examined UExo-derived microRNAs, which reflected the KD status in cats. The examined cats were divided into two groups: normal renal function (NR) and KD. Based on our previous data in clogs and cats, as well as the present data on UExo-derived microRNAs in cats by next-generation sequencing, let-7b, let-7f, miR-10a, miR-10b, miR-21a, miR-22, miR-26a, miR-27b, miR-146a, miR-181a, miR-191, and miR-486a were identified as biomarker candidates. In summary, the levels of UExo-derived let-7b, miR-22, and miR-26a significantly decreased in cats with KD from the early stages of the disease. UExo-derived miRNA levels normalized to urinary creatinine or total RNA of miR-21a was significantly higher in the KD group. Importantly, the ratio of UExo-derived miR-21a to let-7b showed a significant and strongest correlation with serum creatinine (rho = 0.751), blood urea nitrogen (rho = 0.754), and urinary creatinine (rho = -0.421) among all examined indices. Further, the ratio of miR-181a to let-7b or miR-10b significantly correlated with the progression of renal dysfunction in the KD group. Thus, we identified that UExo-derived microRNAs in cats, and their raw and normalized levels could indicate altered renal function. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/72463 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 市居 修
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