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Urinary Exosome-Derived microRNAs Reflecting the Changes in Renal Function in Cats

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Title: Urinary Exosome-Derived microRNAs Reflecting the Changes in Renal Function in Cats
Authors: Ichii, Osamu Browse this author →KAKEN DB
Ohta, Hiroshi Browse this author →KAKEN DB
Horino, Taro Browse this author
Nakamura, Teppei Browse this author
Hosotani, Marina Browse this author
Mizoguchi, Tatsuya Browse this author
Morishita, Keitaro Browse this author →KAKEN DB
Nakamura, Kensuke Browse this author
Sasaki, Noboru Browse this author
Takiguchi, Mitsuyoshi Browse this author →KAKEN DB
Sato, Ryo Browse this author
Oyamada, Kazuhisa Browse this author
Elewa, Yaser Hosny Ali Browse this author →ORCID
Kon, Yasuhiro Browse this author →KAKEN DB
Keywords: urinary exosome-derived miRNA
kidney disease
next-generation sequencing
Issue Date: 20-Nov-2018
Publisher: Frontiers Media
Journal Title: Frontiers in veterinary science
Volume: 5
Start Page: 289
Publisher DOI: 10.3389/fvets.2018.00289
Abstract: Increased incidence of kidney disease (KD) is a common concern in human and companion animals. Cats, in particular, are highly susceptible to KD. Novel KD biomarkers would help to address these problems. Therefore, we are focusing on microRNA, a highly conserved nucleic acid, as a KD biomarker for various animals. We previously reported that altered levels of urinary exosome (UExo)-derived microRNAs indicate renal pathologies in dogs. This study comprehensively examined UExo-derived microRNAs, which reflected the KD status in cats. The examined cats were divided into two groups: normal renal function (NR) and KD. Based on our previous data in clogs and cats, as well as the present data on UExo-derived microRNAs in cats by next-generation sequencing, let-7b, let-7f, miR-10a, miR-10b, miR-21a, miR-22, miR-26a, miR-27b, miR-146a, miR-181a, miR-191, and miR-486a were identified as biomarker candidates. In summary, the levels of UExo-derived let-7b, miR-22, and miR-26a significantly decreased in cats with KD from the early stages of the disease. UExo-derived miRNA levels normalized to urinary creatinine or total RNA of miR-21a was significantly higher in the KD group. Importantly, the ratio of UExo-derived miR-21a to let-7b showed a significant and strongest correlation with serum creatinine (rho = 0.751), blood urea nitrogen (rho = 0.754), and urinary creatinine (rho = -0.421) among all examined indices. Further, the ratio of miR-181a to let-7b or miR-10b significantly correlated with the progression of renal dysfunction in the KD group. Thus, we identified that UExo-derived microRNAs in cats, and their raw and normalized levels could indicate altered renal function.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 市居 修

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