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Specific binding of heterogeneous ribonucleoprotein particle protein K to the human c-myc promoter, in vitro

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/72478

Title: Specific binding of heterogeneous ribonucleoprotein particle protein K to the human c-myc promoter, in vitro
Authors: Takimoto, Masato Browse this author →KAKEN DB
Tomonaga, Takeshi Browse this author
Matunis, Michael Browse this author
Avigan, Mark Browse this author
Krutzsch, Henry Browse this author
Dreyfuss, Gideon Browse this author
Levens, David Browse this author
Issue Date: 25-Aug-1993
Publisher: American Society for Biochemistry and Molecular Biology
Journal Title: The Journal of biological chemistry
Volume: 268
Issue: 24
Start Page: 18249
End Page: 18258
PMID: 08349701
Abstract: A homopurine/homopyrimidine-like sequence is found 100-150 base pairs upstream of the human c-myc promoter P1. This element, termed the CT-element, has been shown to augment expression from P1, and it serves as a positive transcriptional element when coupled to a heterologous promoter in vivo and in vitro. Synthetic oligonucleotides comprising this element were used to form DNA-protein complexes in electrophoretic mobility shift assays. By using conventional and affinity methods, 61- and 34-kDa proteins were shown to be associated with these complexes. Amino acid sequence analysis and immunological methods have identified these proteins as heterogeneous ribonucleoprotein particle (hnRNP) proteins K and A1. Surprisingly, hnRNP protein K binds to the pyrimidine-rich strand of the CT-element in a sequence-specific manner as well as to the double-stranded molecule. Cotransfection of vectors encoding hnRNP protein K in the sense or anti-sense orientations with reporter plasmids driven by wild-type or mutant CT-elements demonstrates that hnRNP protein K augments gene expression in a cis-element-dependent manner. Taken together, these results suggest that hnRNP protein K may play a role in the transcriptional regulation of the human c-myc gene.
Rights: This research was originally published in the Journal of Biological Chemistry. M Takimoto, T Tomonaga, M Matunis, M Avigan, H Krutzsch, G Dreyfuss and D Levens. Specific binding of heterogeneous ribonucleoprotein particle protein K to the human c-myc promoter, in vitro. J. Biol. Chem. 1993; 268:18249-18258. © the American Society for Biochemistry and Molecular Biology.
Relation: http://www.jbc.org/content/268/24/18249.abstract
Type: article
URI: http://hdl.handle.net/2115/72478
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 瀧本 将人

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