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Specific binding of heterogeneous ribonucleoprotein particle protein K to the human c-myc promoter, in vitro
| Title: | Specific binding of heterogeneous ribonucleoprotein particle protein K to the human c-myc promoter, in vitro |
| Authors: | Takimoto, Masato Browse this author →KAKEN DB | | Tomonaga, Takeshi Browse this author | | Matunis, Michael Browse this author | | Avigan, Mark Browse this author | | Krutzsch, Henry Browse this author | | Dreyfuss, Gideon Browse this author | | Levens, David Browse this author |
| Issue Date: | 25-Aug-1993 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Journal Title: | The Journal of biological chemistry |
| Volume: | 268 |
| Issue: | 24 |
| Start Page: | 18249 |
| End Page: | 18258 |
| PMID: | 8349701 |
| Abstract: | A homopurine/homopyrimidine-like sequence is found 100-150 base pairs upstream of the human c-myc promoter P1. This element, termed the CT-element, has been shown to augment expression from P1, and it serves as a positive transcriptional element when coupled to a heterologous promoter in vivo and in vitro. Synthetic oligonucleotides comprising this element were used to form DNA-protein complexes in electrophoretic mobility shift assays. By using conventional and affinity methods, 61- and 34-kDa proteins were shown to be associated with these complexes. Amino acid sequence analysis and immunological methods have identified these proteins as heterogeneous ribonucleoprotein particle (hnRNP) proteins K and A1. Surprisingly, hnRNP protein K binds to the pyrimidine-rich strand of the CT-element in a sequence-specific manner as well as to the double-stranded molecule. Cotransfection of vectors encoding hnRNP protein K in the sense or anti-sense orientations with reporter plasmids driven by wild-type or mutant CT-elements demonstrates that hnRNP protein K augments gene expression in a cis-element-dependent manner. Taken together, these results suggest that hnRNP protein K may play a role in the transcriptional regulation of the human c-myc gene. |
| Rights: | This research was originally published in the Journal of Biological Chemistry. M Takimoto, T Tomonaga, M Matunis, M Avigan, H Krutzsch, G Dreyfuss and D Levens. Specific binding of heterogeneous ribonucleoprotein particle protein K to the human c-myc promoter, in vitro. J. Biol. Chem. 1993; 268:18249-18258. © the American Society for Biochemistry and Molecular Biology. |
| Relation: | http://www.jbc.org/content/268/24/18249.abstract |
| Type: | article |
| URI: | http://hdl.handle.net/2115/72478 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 瀧本 将人
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