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Hokkaido University Collection of Scholarly and Academic Papers >
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Impact of capsaicin, an active component of chili pepper, on pathogenic chlamydial growth (Chlamydia trachomatis and Chlamydia pneumoniae) in immortal human epithelial HeLa cells
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| Title: | Impact of capsaicin, an active component of chili pepper, on pathogenic chlamydial growth (Chlamydia trachomatis and Chlamydia pneumoniae) in immortal human epithelial HeLa cells |
| Authors: | Yamakawa, Kazuya Browse this author | | Matsuo, Junji Browse this author →KAKEN DB | | Okubo, Torahiko Browse this author | | Nakamura, Shinji Browse this author →KAKEN DB | | Yamaguchi, Hiroyuki Browse this author →KAKEN DB |
| Keywords: | Chlamydia trachomatis | | Capsaicin | | HeLa cells | | TRPV1 | | PPAR gamma | | LXR alpha |
| Issue Date: | Feb-2018 |
| Publisher: | Elsevier |
| Journal Title: | Journal of infection and chemotherapy |
| Volume: | 24 |
| Issue: | 2 |
| Start Page: | 130 |
| End Page: | 137 |
| Publisher DOI: | 10.1016/j.jiac.2017.10.007 |
| PMID: | 29132924 |
| Abstract: | Chlamydia trachomatis is the leading cause of sexually transmitted infections worldwide. Capsaicin, a component of chili pepper, which can stimulate actin remodeling via capsaicin receptor TRPV1 (transient receptor potential vanilloid 1) and anti-inflammatory effects via PPAR gamma (peroxisome proliferator-activated receptor-gamma) and LXR alpha (liver X receptor alpha), is a potential candidate to control chlamydial growth in host cells. We examined whether capsaicin could inhibit C. trachomatis growth in immortal human epithelial HeLa cells. Inclusion forming unit and quantitative PCR assays showed that capsaicin significantly inhibited bacterial growth in cells in a dose-dependent manner, even in the presence of cycloheximide, a eukaryotic protein synthesis inhibitor. Confocal microscopic and transmission electron microscopic observations revealed an obvious decrease in bacterial numbers to inclusions bodies formed in the cells. Although capsaicin can stimulate the apoptosis of cells, no increase in cleaved PARP (poly (ADP-ribose) polymerase), an apoptotic indicator, was observed at a working concentration. All of the drugs tested (capsazepine, a TRPV1 antagonist; 5CPPSS-50, an LXR alpha inhibitor; and T0070907, a PPAR gamma inhibitor) had no effect on chlamydial inhibition in the presence of capsaicin. In addition, we also confirmed that capsaicin inhibited Chlamydia pneumoniae growth, indicating a phenomena not specific to C. trachomatis. Thus, we conclude that capsaicin can block chlamydial growth without the requirement of host cell protein synthesis, but by another, yet to be defined, mechanism. |
| Rights: | © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/72706 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山口 博之
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