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Volume 67 Number 1 >

Evaluation of immunohistochemical staining with PMab-38, an anti-dog podoplanin monoclonal antibody, in various canine tumor tissues

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Title: Evaluation of immunohistochemical staining with PMab-38, an anti-dog podoplanin monoclonal antibody, in various canine tumor tissues
Authors: Kiname, Kohei Browse this author
Yoshimoto, Sho Browse this author
Kato, Daiki Browse this author
Tsuboi, Masaya Browse this author
Tanaka, Yuiko Browse this author
Yoshitake, Ryohei Browse this author
Eto, Shotaro Browse this author
Shinada, Masahiro Browse this author
Chambers, James Browse this author
Saeki, Kohei Browse this author
Kinoshita, Ryohei Browse this author
Yamada, Shinji Browse this author
Uchida, Kazuyuki Browse this author →KAKEN DB
Kaneko, Mika K. Browse this author
Nishimura, Ryohei Browse this author →KAKEN DB
Kato, Yukinari Browse this author
Nakagawa, Takayuki Browse this author →KAKEN DB
Keywords: podoplanin
canine cancer
Issue Date: Feb-2019
Publisher: Faculty of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 67
Issue: 1
Start Page: 25
End Page: 34
Abstract: Podoplanin (PDPN) is a type I transmembrane sialoglycoprotein with O-glycosylation and a high content of sialic acid. PDPN has been reported to be expressed in various human tumors and promote tumor progression, epithelial-mesenchymal transition, and distant metastasis. PDPN is also expressed in cancer-associated fibroblasts (CAFs), which promote tumor growth. We have developed novel tumor specific anti-PDPN antibodies. PMab-38, which is an anti-dog PDPN monoclonal antibody, recognized PDPN expression in canine squamous cell carcinoma (SCC) and malignant melanoma. However, there has been no research into PMab-38 recognition of other types of tumors and systemic normal tissue. The objective of this study was to evaluate the staining positivity of PMab-38 by immunohistochemical staining of various paraffin-embedded canine tumor and systemic normal tissues. Immunohistochemical analysis revealed that PMab-38 positively stained tumor cells in 9/11 (82%) SCC and 9/11 (82%) pulmonary adenocarcinoma tissues. In the tumor stroma, large spindle-shaped mesenchymal cells, which were suspected to be CAFs, were stained by PMab-38 in almost all tumor types: 9/10 (90%) for anal sac adenocarcinoma and 11/12 (92%) for transitional cell carcinoma tissues. Almost all normal tissues were negatively stained with PMab-38 except part of the kidney glomerulus. Taken together, our findings provide evidence that various types of tumor cells were strongly stained by PMab-38, but most normal cells were not stained. These results indicate that PDPN recognized by PMab-38 might be a target for tumor antigen targeting therapy. Further studies are required to investigate the anti-tumor effect in various canine tumors by antibody therapy using PMab-38.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 67 Number 1

Submitter: 獣医学部図書室

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