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beta-carotene and retinol reduce benzo[a]pyrene-induced mutagenicity and oxidative stress via transcriptional modulation of xenobiotic metabolizing enzymes in human HepG2 cell line

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/72739

Title: beta-carotene and retinol reduce benzo[a]pyrene-induced mutagenicity and oxidative stress via transcriptional modulation of xenobiotic metabolizing enzymes in human HepG2 cell line
Authors: Darwish, Wageh Sobhy Browse this author
Ikenaka, Yoshinori Browse this author →KAKEN DB
Nakayama, Shouta Browse this author
Mizukawa, Hazuki Browse this author →KAKEN DB
Thompson, Lesa A. Browse this author
Ishizuka, Mayumi Browse this author →KAKEN DB
Keywords: Carotenoids
Retinoids
B[a]P
Mutagenicity
Oxidative stress
HepG2 cells
Diet
Pollution
Issue Date: Mar-2018
Publisher: Springer
Journal Title: Environmental science and pollution research
Volume: 25
Issue: 7
Start Page: 6320
End Page: 6328
Publisher DOI: 10.1007/s11356-017-0977-z
PMID: 29247416
Abstract: Benzo[a]pyrene (B[a]P) is one of the polycyclic aromatic hydrocarbons which is formed due to smoking of foods, incomplete combustion of woods, vehicle exhausts, and cigarettes smokes. B[a]P gets entry into human and animal bodies mainly through their diets. Metabolic activation of B[a]P is required to induce mutagenesis and carcinogenesis in animal and human studies. Carotenoids and retinoids are phytochemicals that if ingested have multiple physiological interferences in the human and animal bodies. In this study, we firstly investigated the protective effects of beta-carotene, beta-apo-8-carotenal, retinol, and retinoic acid against B[a]P-induced mutagenicity and oxidative stress in human HepG2 cells. Secondly, we tested the hypothesis of modulating xenobiotic metabolizing enzymes (XMEs) by carotenoids and retinoids as a possible mechanism of protection by these micronutrients against B[a]P adverse effects. The obtained results declared that beta-carotene and retinol significantly reduced B[a]P-induced mutagenicity and oxidative stress. Tested carotenoids and retinoids reduced B[a]P-induced phase I XMEs and induced B[a]P reduced phase II and III XMEs. Thus, the protective effects of these micronutrients are probably due to their ability of induction of phase II and III enzymes and interference with the induction of phase I enzymes by the promutagen, B[a]P. It is highly recommended to consume foods rich in these micronutrients in the areas of high PAH pollution.
Rights: The final publication is available at link.springer.com
Type: article (author version)
URI: http://hdl.handle.net/2115/72739
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石塚 真由美

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