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Volume 67 Number 1 >

Real-time PCR assay for rapid differentiation of env-based genotypes of feline leukemia virus

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Title: Real-time PCR assay for rapid differentiation of env-based genotypes of feline leukemia virus
Authors: Nakagawa, Shihono Browse this author
Kitamura, Yu Browse this author
Naito, Ikunori Browse this author
Kaneda, Masahiko Browse this author
Chiba, Yusuke Browse this author
Shimamura, Shunsuke Browse this author
Yamasaki, Masahiro Browse this author →KAKEN DB
Hikono, Hirokazu Browse this author →KAKEN DB
Murakami, Kenji Browse this author
Keywords: Feline leukemia virus
real-time PCR
Issue Date: Feb-2019
Publisher: Faculty of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 67
Issue: 1
Start Page: 103
End Page: 109
Abstract: The envelope protein plays an essential role in the pathogenesis of feline leukemia virus (FeLV). Three categories of genotypes have been identified according to the sequence of the envelope gene (env). The relationship between env-based genotype and viral pathogenicity remains to be investigated. However, this effort is hampered by the fact that sequencing and phylogenetic analysis of env are expensive and time-consuming. In this study, we identified single-nucleotide polymorphisms (SNPs) that are located in the long terminal repeat but linked to env-based genotypes (G), I and III. Our real-time PCR assay included a primer containing the SNP sites and detected GI proviruses but not GIII proviruses. It will help rapid differentiation of the env-based genotypes of FeLV field isolates.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 67 Number 1

Submitter: 獣医学部図書室

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