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Molecular characterization and gene expression of synaptosome-associated protein-25 (SNAP-25) in the brain during both seaward and homeward migrations of chum salmon Oncorhynchus keta
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| Title: | Molecular characterization and gene expression of synaptosome-associated protein-25 (SNAP-25) in the brain during both seaward and homeward migrations of chum salmon Oncorhynchus keta |
| Authors: | Abe, Takashi Browse this author | | Minowa, Yui Browse this author | | Kudo, Hideaki Browse this author →KAKEN DB |
| Keywords: | Neurotransmission | | Olfactory imprinting | | Salmon | | Migration | | Pre-synaptic exocytosis | | SNAP-25 | | Teleostei |
| Issue Date: | Mar-2018 |
| Publisher: | Elsevier |
| Journal Title: | Comparative Biochemistry and Physiology Part A : molecular & integrative physiology |
| Volume: | 217 |
| Start Page: | 17 |
| End Page: | 25 |
| Publisher DOI: | 10.1016/j.cbpa.2017.12.006 |
| PMID: | 29248569 |
| Abstract: | It is generally accepted that information about some of the odorants in the natal streams of anadromous Pacific salmon (Genus Oncorhynchus) is imprinted during their seaward migration, and that anadromous Pacific salmon use olfaction to identify their natal streams during the homeward migration. However, little is known about the molecular mechanisms of the various pre-synaptic functions that are important for olfactory imprinting and memory retrieval in the salmon brain. Synaptosome-associated protein-25 (SNAP-25) mediates pre-synaptic vesicle exocytosis and regulates synaptic transmission and neuronal plasticity. Despite the importance of synaptic plasticity for memorization, the expression of SNAP-25 in the salmon brain is not well understood. In this study, snap25 expression was detected in chum salmon (O. keta) brains using molecular biological techniques. Two cDNAs encoding salmon SNAP-25 were isolated and sequenced (SNAP-25a and SNAP-25b). These cDNAs encoded proteins with 204 amino acid residues, which showed marked homology with each other (97%). The protein and nucleotide sequences demonstrated a high level of homology between salmon SNAP-25s and those of other teleost species. By quantitative PCR, the expression of snap25a and snap25b was detected in all regions of the salmon brain, especially in the telencephalon. The expression levels of snap25a in the olfactory blub were higher during seaward migration than in upriver and post-upriver migrations, reflecting synaptogenesis in the olfactory nervous system, and snap25b in the telencephalon was increased during upriver period. Our results indicated that snap25s gene is involved in synaptic plasticity for olfactory imprinting and/or olfactory memory retrieval in Pacific salmon. |
| Rights: | © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/72799 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 工藤 秀明
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