Title: | The Role of Heparan Sulfate Proteoglycans as an Attachment Factor for Rabies Virus Entry and Infection |
Authors: | Sasaki, Michihito Browse this author →KAKEN DB |
Anindita, Paulina D. Browse this author |
Ito, Naoto Browse this author |
Sugiyama, Makoto Browse this author |
Carr, Michael Browse this author |
Fukuhara, Hideo Browse this author |
Ose, Toyoyuki Browse this author →KAKEN DB |
Maenaka, Katsumi Browse this author →KAKEN DB |
Takada, Ayato Browse this author →KAKEN DB |
Hall, William W. Browse this author |
Orba, Yasuko Browse this author →KAKEN DB |
Sawa, Hirofumi Browse this author →KAKEN DB |
Keywords: | rabies virus |
heparan sulfate |
viral attachment |
viral glycoprotein |
host-virus interaction |
Issue Date: | 26-Feb-2018 |
Publisher: | Oxford University Press |
Journal Title: | The Journal of infectious diseases |
Volume: | 217 |
Issue: | 11 |
Start Page: | 1740 |
End Page: | 1749 |
Publisher DOI: | 10.1093/infdis/jiy081 |
PMID: | 29529215 |
Abstract: | Rabies virus (RABV) is the causative agent of fatal neurological disease. Cellular attachment is the initial and essential step for viral infections. Although extensive studies have demonstrated that RABV uses various target cell molecules to mediate infection, no specific molecule has been identified as an attachment factor for RABV infection. Here we demonstrate that cellular heparan sulfate (HS) supports RABV adhesion and subsequent entry into target cells. Enzymatic removal of HS reduced cellular susceptibility to RABV infection, and heparin, a highly sulfated form of HS, blocked viral adhesion and infection. The direct binding between RABV glycoprotein and heparin was demonstrated, and this interaction was shown to require HS N- and 6-O-sulfation. We also revealed that basic amino acids in the ectodomain of RABV glycoprotein serve as major determinants for the RABV-HS interaction. Collectively, our study highlights a previously undescribed role of HS as an attachment factor for RABV infection. |
Rights: | This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Infectious Diseases following peer review. The version of record [Volume 217, Issue 11, 5 May 2018] is available online at: https://doi.org/10.1093/infdis/jiy081. |
Relation: | 出版社版へのリンク |
https://academic.oup.com/jid/article/217/11/1740/4909726 |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/72895 |
Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc) 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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