HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Research Center for Zoonosis Control >
Peer-reviewed Journal Articles, etc >

The Role of Heparan Sulfate Proteoglycans as an Attachment Factor for Rabies Virus Entry and Infection

Files in This Item:
Clean_Sup_figure_legends.docxSupplementary_legends54.14 kBMicrosoft Word XMLView/Open
Clean_Sup_methods R1.docxSupplementary_methods53.78 kBMicrosoft Word XMLView/Open
JID.pdf論文本体1.75 MBPDFView/Open
Supplementary_FigS1 R1.tifSupplementary_FigS1224.4 kBTIFFView/Open
Supplementary_FigS2.tifSupplementary_FigS2113.24 kBTIFFView/Open
Supplementary_FigS3.tifSupplementary_FigS3107.66 kBTIFFView/Open
Supplementary_FigS4.tifSupplementary_FigS4356.18 kBTIFFView/Open
Supplementary_FigS5 R1.tifSupplementary_FigS5139.14 kBTIFFView/Open
Supplementary_FigS6 R1.tifSupplementary_FigS6142.71 kBTIFFView/Open
Supplementary_FigS7.tifSupplementary_FigS7250.24 kBTIFFView/Open
Supplementary_FigS8 R1.tifSupplementary_FigS8387.07 kBTIFFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/72895

Title: The Role of Heparan Sulfate Proteoglycans as an Attachment Factor for Rabies Virus Entry and Infection
Authors: Sasaki, Michihito Browse this author →KAKEN DB
Anindita, Paulina D. Browse this author
Ito, Naoto Browse this author
Sugiyama, Makoto Browse this author
Carr, Michael Browse this author
Fukuhara, Hideo Browse this author
Ose, Toyoyuki Browse this author →KAKEN DB
Maenaka, Katsumi Browse this author →KAKEN DB
Takada, Ayato Browse this author →KAKEN DB
Hall, William W. Browse this author
Orba, Yasuko Browse this author →KAKEN DB
Sawa, Hirofumi Browse this author →KAKEN DB
Keywords: rabies virus
heparan sulfate
viral attachment
viral glycoprotein
host-virus interaction
Issue Date: 26-Feb-2018
Publisher: Oxford University Press
Journal Title: The Journal of infectious diseases
Volume: 217
Issue: 11
Start Page: 1740
End Page: 1749
Publisher DOI: 10.1093/infdis/jiy081
PMID: 29529215
Abstract: Rabies virus (RABV) is the causative agent of fatal neurological disease. Cellular attachment is the initial and essential step for viral infections. Although extensive studies have demonstrated that RABV uses various target cell molecules to mediate infection, no specific molecule has been identified as an attachment factor for RABV infection. Here we demonstrate that cellular heparan sulfate (HS) supports RABV adhesion and subsequent entry into target cells. Enzymatic removal of HS reduced cellular susceptibility to RABV infection, and heparin, a highly sulfated form of HS, blocked viral adhesion and infection. The direct binding between RABV glycoprotein and heparin was demonstrated, and this interaction was shown to require HS N- and 6-O-sulfation. We also revealed that basic amino acids in the ectodomain of RABV glycoprotein serve as major determinants for the RABV-HS interaction. Collectively, our study highlights a previously undescribed role of HS as an attachment factor for RABV infection.
Rights: This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Infectious Diseases following peer review. The version of record [Volume 217, Issue 11, 5 May 2018] is available online at: https://doi.org/10.1093/infdis/jiy081.
Relation: 出版社版へのリンク
https://academic.oup.com/jid/article/217/11/1740/4909726
Type: article (author version)
URI: http://hdl.handle.net/2115/72895
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐々木 道仁

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

Feedback - Hokkaido University