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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
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Na,K-ATPase in dog red cells. Immunological identification and maturation-associated degradation by the proteolytic system.
| Title: | Na,K-ATPase in dog red cells. Immunological identification and maturation-associated degradation by the proteolytic system. |
| Authors: | Inaba, Mutsumi Browse this author →KAKEN DB | | Maede, Yoshimitsu Browse this author →KAKEN DB |
| Issue Date: | 5-Dec-1986 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Journal Title: | The Journal of biological chemistry |
| Volume: | 261 |
| Issue: | 34 |
| Start Page: | 16099 |
| End Page: | 16105 |
| PMID: | 3023340 |
| Abstract: | The Na,K-ATPase of red cells from high K+ and low K+ dogs was studied immunologically by using antibodies raised against dog kidney enzyme. Anti-alpha subunit IgGs, which also recognized alpha (+) from brain enzyme, identified the larger subunit of erythrocyte Na,K-ATPase as a homogeneous polypeptide with Mr = 96,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by immunoblotting. In addition, erythrocyte Na,K-ATPase, purified by immunoaffinity chromatography on a monoclonal antibody-coupled column, showed the identity of its polypeptide composition to that of the renal enzyme. Furthermore, it was shown that reticulocyte lysates from high K+ and low K+ dogs substantially degraded 125I-Bolton-Hunter reagent-labeled Na,K-ATPase. This degradation of the enzyme protein was significantly enhanced by the addition of ATP and Mg2+. These results indicate that dog reticulocytes possess some mechanism for protein breakdown involving an ATP-dependent proteolytic system, resulting in the dramatic breakdown of Na,K-ATPase activity during dog reticulocyte maturation into erythrocytes (Maede, Y., and Inaba, M. (1985) J. Biol. Chem. 260, 3337-3343). |
| Relation: | http://www.jbc.org/content/261/34/16099 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/73328 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 前出 吉光
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