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Relationship between the antral follicle count in bovine ovaries from a local abattoir and steroidogenesis of granulosa cells cultured as oocyte-cumulus-granulosa complexes

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Title: Relationship between the antral follicle count in bovine ovaries from a local abattoir and steroidogenesis of granulosa cells cultured as oocyte-cumulus-granulosa complexes
Authors: Sakaguchi, Kenichiro Browse this author
Tanida, Takashi Browse this author
Abdel-Ghani, Mohammed A. Browse this author
Kanno, Chihiro Browse this author
Yanagawa, Yojiro Browse this author
Katagiri, Seiji Browse this author →KAKEN DB
Nagano, Masashi Browse this author →KAKEN DB
Keywords: Antral follicle count
Bovine oocyte
In vitro growth
Steroidogenesis
Issue Date: Dec-2018
Publisher: Society for Reproduction and Development(SRD)
Journal Title: Journal of reproduction and development
Volume: 64
Issue: 6
Start Page: 503
End Page: 510
Publisher DOI: 10.1262/jrd.2018-080
Abstract: The antral follicle count (AFC) is used as an indicator of cow fertility. We herein investigated the relationship between AFC and the steroidogenesis of granulosa cells and confirmed the developmental competence of oocytes derived from early antral follicles (0.5-1.0 mm) using in vitro growth culture. Slaughterhouse -derived ovaries were divided into high (>= 25) and low (< 25) AFC groups based on AFC (>= 2.0 mm). Oocyte-cumulus-granulosa complexes (OCGC5) collected from early antral follicles were cultured for 12 days. The total number, viability, and diameter of granulosa cells and estradiol-17 beta and progesterone production during the culture were evaluated. Surviving oocytes on day 12 were subjected to in vitro maturation, and their volume and nuclear status were evaluated. Some oocytes were subjected to the evaluation of developmental competence to blastocysts. Although the total number and viability of granulosa cells did not differ between the groups, granulosa cell diameters were smaller in the high AFC group than in the low AFC group. The estradiol-17 beta and progesterone ratio on day 8 was higher in the high AFC group than in the low AFC group. Oocyte volumes and nuclear maturation rates were greater in the high AFC group than in the low AFC group. The development rate to blastocysts was 9.1% in the high AFC group, while no oocytes developed to blastocysts in the low AFC group. Therefore, estradiol-17 beta production by granulosa cells appears to be greater in high AFC cattle than in low AFC cattle, thereby promoting the acquisition of oocyte competence.
Rights: http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article
URI: http://hdl.handle.net/2115/73353
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永野 昌志

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