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Control of elicitor-induced oxidative burst by abscisic acid associated with growth of Saccharina japonica (Phaeophyta, Laminariales) sporophytes
| Title: | Control of elicitor-induced oxidative burst by abscisic acid associated with growth of Saccharina japonica (Phaeophyta, Laminariales) sporophytes |
| Authors: | Shimizu, Kazuki Browse this author | | Uji, Toshiki Browse this author →KAKEN DB | | Yasui, Hajime Browse this author →KAKEN DB | | Mizuta, Hiroyuki Browse this author →KAKEN DB |
| Keywords: | Abscisic acid | | Elicitor | | Growth | | lodoperoxidase | | Kelp | | Oxidative burst |
| Issue Date: | Apr-2018 |
| Publisher: | Springer |
| Journal Title: | Journal of Applied Phycology |
| Volume: | 30 |
| Issue: | 2 |
| Start Page: | 1371 |
| End Page: | 1379 |
| Publisher DOI: | 10.1007/s10811-017-1320-2 |
| Abstract: | In this study, the oligoguluronate elicitor-induced oxidative burst (OB) was monitored continuously in young and mature Saccharina japonica sporophytes based on luminol chemiluminescence using a photon counter. The iodoperoxidase (IPO) activity, abscisic acid (ABA), and polyphenol contents were also compared in the different growth stages. The elicitor-induced OB occurred within 1 min and reached its maximum in 15-20 min after treatment in all growth stages. The active elicitor-induced OB was stronger in the young sporophytes than the older sporophytes. The IPO activity in the different growth stages also exhibited a similar pattern to the elicitor-induced OB. These results suggest that the elicitor-induced OB and the subsequent high haloperoxidase activity comprise a major defence mechanism in young sporophytes. By contrast, ABA accumulated with the growth of the sporophytes. Interestingly, ABA treatment suppressed the elicitor-induced OB during growth and enhanced the elicitor-independent IPO activity even in the young sporophytes. In addition, the polyphenol content was higher in the older sporophytes than the younger sporophytes. These observations show that dramatic changes occur in the characteristic defences against biotic stresses as the sporophyte grows, as well as suggesting that ABA is closely linked with these changes. Moreover, the IPO activity recovered slightly in the sorus, which is the reproductive tissue, thereby suggesting that a higher ABA content increases the defence activity and the success of reproduction. |
| Rights: | This is a post-peer-review, pre-copyedit version of an article published in Journal of Applied Phycology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10811-017-1320-2 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/73421 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 水田 浩之
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