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Identification and analysis of host proteins that interact with the 3 '-untranslated region of tick-borne encephalitis virus genomic RNA

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Title: Identification and analysis of host proteins that interact with the 3 '-untranslated region of tick-borne encephalitis virus genomic RNA
Authors: Muto, Memi Browse this author
Kamitani, Wataru Browse this author
Sakai, Mizuki Browse this author
Hirano, Minato Browse this author
Kobayashi, Shintaro Browse this author →KAKEN DB
Kariwa, Hiroaki Browse this author →KAKEN DB
Yoshii, Kentaro Browse this author →KAKEN DB
Keywords: Tick-borne encephalitis virus
3 '-Untranslated region
Host factor
Pathogenicity
Issue Date: 2-Apr-2018
Publisher: Elsevier
Journal Title: Virus Research
Volume: 249
Start Page: 52
End Page: 56
Publisher DOI: 10.1016/j.virusres.2018.03.006
PMID: 29545014
Abstract: Tick-borne encephalitis virus (TBEV) causes severe neurological disease, but the pathogenetic mechanism is unclear. The conformational structure of the 3'-untranslated region (UTR) of TBEV is associated with its virulence. We tried to identify host proteins interacting with the 3'-UTR of TBEV. Cellular proteins of HEK293T cells were co-precipitated with biotinylated RNAs of the 3'-UTR of low-and high-virulence TBEV strains and subjected to mass spectrometry analysis. Fifteen host proteins were found to bind to the 3'-UTR of TBEV, four of which cold shock domain containing-El (CSDE1), spermatid perinuclear RNA binding protein (STRBP), fragile X mental retardation protein (FMRP), and interleukin enhancer binding factor 3 (ILF3)-bound specifically to that of the low-virulence strain. An RNA immunoprecipitation and pull-down assay confirmed the interactions of the complete 3'-UTRs of TBEV genomic RNA with CSDE1, FMRP, and ILF3. Partial deletion of the stem loop (SL) 3 to SL 5 structure of the variable region of the 3'-UTR did not affect interactions with the host proteins, but the interactions were markedly suppressed by deletion of the complete SL 3, 4, and 5 structures, as in the high virulence TBEV strain. Further analysis of the roles of host proteins in the neurologic pathogenicity of TBEV is warranted.
Rights: http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/73473
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 好井 健太朗

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