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Preventive effect of mesenchymal stem cell culture supernatant on luminal stricture after endoscopic submucosal dissection in the rectum of pigs

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/73707

Title: Preventive effect of mesenchymal stem cell culture supernatant on luminal stricture after endoscopic submucosal dissection in the rectum of pigs
Authors: Tsuda, Momoko Browse this author
Ohnishi, Shunsuke Browse this author →KAKEN DB
Mizushima, Takeshi Browse this author
Hosono, Hidetaka Browse this author
Yamahara, Kenichi Browse this author →KAKEN DB
Ishikawa, Marin Browse this author
Abiko, Satoshi Browse this author
Katsurada, Takehiko Browse this author
Shimizu, Yuichi Browse this author →KAKEN DB
Sakamoto, Naoya Browse this author →KAKEN DB
Issue Date: Oct-2018
Publisher: Georg Thieme Verlag
Journal Title: Endoscopy
Volume: 50
Issue: 10
Start Page: 1001
End Page: 1016
Publisher DOI: 10.1055/a-0584-7262
Abstract: Background: Mesenchymal stem cells (MSCs) are valuable in regenerative medicine, and MSC culture supernatant (MSC-CS) reportedly inhibits inflammation and fibrosis. We investigated whether colorectal luminal stricture develops after circumferential endoscopic submucosal dissection (ESD) in the colorectum, and whether the development of luminal stricture could be prevented by using MSC-CS enema. Methods: In the first experiment, we performed circumferential ESD in the rectums or distal colons of pigs (n = 4 in each group). We sacrificed the pigs on Day 22 and measured the degree of luminal stricture. In the second experiment, we performed circumferential ESD in the rectums of pigs and administered an MSC-CS gel or a control gel enema after ESD for 4 days. We sacrificed the pigs on Day 8 (n = 3 in each group) or 22 (n = 3 in each group) to measure the degree of luminal stricture, and performed histological analysis. Results: Severe luminal stricture was observed in the rectum but not in the distal colon. Moreover, fiber accumulation in the submucosa and hypertrophy of the muscularis propria were observed in the rectum but not in the distal colon. The degree of luminal stricture in the rectum was significantly lower in the MSC-CS group than in the control group. Furthermore, MSC-CS attenuated myofibroblast activation and hypertrophy of the muscularis propria on Day 22, and reduced inflammatory cell infiltration on Day 8. Conclusions: Luminal stricture after ESD developed only in the rectum because of the difference in myofibroblast activation and fiber accumulation. In addition, MSC-CS enema prevented luminal stricture after ESD, possibly by inhibiting the inflammatory reaction and fibrosis.
Rights: This is an Accepted Manuscript of an article published by Thieme Publishing Group in Endoscopy on October 2018, available online at https://www.thieme-connect.de/products/ejournals/abstract/10.1055/a-0584-7262
Type: article (author version)
URI: http://hdl.handle.net/2115/73707
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 大西 俊介

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