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Amoebal endosymbiont Neochlamydia protects host amoebae against Legionella pneumophila infection by preventing Legionella entry

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Title: Amoebal endosymbiont Neochlamydia protects host amoebae against Legionella pneumophila infection by preventing Legionella entry
Authors: Maita, Chinatsu Browse this author
Matsushita, Mizue Browse this author
Miyoshi, Masahiro Browse this author
Okubo, Torahiko Browse this author →KAKEN DB
Nakamura, Shinji Browse this author →KAKEN DB
Matsuo, Junji Browse this author →KAKEN DB
Takemura, Masaharu Browse this author
Miyake, Masaki Browse this author
Nagai, Hiroki Browse this author
Yamaguchi, Hiroyuki Browse this author →KAKEN DB
Keywords: Symbiosis
Issue Date: Apr-2018
Publisher: Elsevier
Journal Title: Microbes and Infection
Volume: 20
Issue: 4
Start Page: 236
End Page: 244
Publisher DOI: 10.1016/j.micinf.2017.12.012
Abstract: Acanthamoeba isolated from environmental soil harbors the obligate intracellular symbiont Neochlamydia, which has a critical role in host amoebal defense against Legionella pneumophila infection. Here, by using morphological analysis with confocal laser scanning fluorescence microscopy and transmission electron microscopy, proteome analyses with two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and liquid chromatography–mass spectrometry (LC/MS), and transcriptome analysis with DNA microarray, we explored the mechanism by which the Neochlamydia effected this defense. We observed that when rare uptake did occur, the symbiotic amoebae allowed Legionella to grow normally. However, the symbiotic amoebae had severely reduced uptake of Legionella when compared with the aposymbiotic amoebae. Also, in contrast to amoebae carrying the endosymbiont, the actin cytoskeleton was significantly disrupted by Legionella infection in aposymbiotic amoebae. Furthermore, despite Legionella exposure, there was little change in Neochlamydia gene expression. Taken together, we concluded that the endosymbiont, Neochlamydia prevents Legionella entry to the host amoeba, resulting in the host defense against Legionella infection.
Rights: © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 博之

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