Title: | Discovery of Mwinilunga alphavirus : A novel alphavirus in Culex mosquitoes in Zambia |
Authors: | Torii, Shiho Browse this author |
Orba, Yasuko Browse this author →KAKEN DB |
Hang'ombe, Bernard M. Browse this author |
Mweene, Aaron S. Browse this author |
Wada, Yuji Browse this author |
Anindita, Paulina D. Browse this author |
Phongphaew, Wallaya Browse this author |
Qiu, Yongjin Browse this author |
Kajihara, Masahiro Browse this author |
Mori-Kajihara, Akina Browse this author |
Eto, Yoshiki Browse this author |
Harima, Hayato Browse this author |
Sasaki, Michihito Browse this author |
Carr, Michael Browse this author |
Hall, William W. Browse this author |
Eshita, Yuki Browse this author |
Abe, Takashi Browse this author |
Sawa, Hirofumi Browse this author |
Keywords: | Alphavirus |
Mosquito |
Culex |
Zambia |
Issue Date: | 2-May-2018 |
Publisher: | Elsevier |
Journal Title: | Virus Research |
Volume: | 250 |
Start Page: | 31 |
End Page: | 36 |
Publisher DOI: | 10.1016/j.virusres.2018.04.005 |
Abstract: | Mosquito-borne alphaviruses are disseminated globally and cause febrile illness in humans and animals. Since the prevalence and diversity of alphaviruses has not been previously investigated in Zambia, reverse transcription PCR was employed as a broad-spectrum approach for the detection of alphaviruses in mosquitoes. From 552 mosquito pools, a novel alphavirus, tentatively named Mwinilunga alphavirus (MWAV), was discovered from a single Culex quinquefasciatus mosquito pool. The full genome of MWAV was subsequently determined, and pairwise comparisons demonstrated that MWAV represented a new alphavirus species. Phylogenetic analyses and a linear discriminant analysis based on the dinucleotide ratios in various virus sequences indicated that MWAV is related to a mosquito-specific alphavirus distinct from other known mosquito-borne alphaviruses due to its inability to replicate in vertebrate cell lines. Further analyses of these novel alphaviruses will help to facilitate a greater understanding of the molecular determinants of host range restriction and the evolutionary relationships of alphaviruses. |
Rights: | © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/73890 |
Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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