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Evaluation of an aphid-rearing method using excised leaves and agar medium

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Title: Evaluation of an aphid-rearing method using excised leaves and agar medium
Authors: Li, Yang Browse this author
Akimoto, Shin-ichi Browse this author →KAKEN DB
Keywords: Acyrthosiphon pisum
developmental time
sexual generation
Vicia faba
Issue Date: Jun-2018
Publisher: John Wiley & Sons
Journal Title: Entomological science
Volume: 21
Issue: 2
Start Page: 210
End Page: 215
Publisher DOI: 10.1111/ens.12296
Abstract: The present study evaluated the effectiveness of an aphid-rearing method devised by Milner in 1981 using Acyrthosiphon pisum and its host plant Vicia faba. In the agar-leaf method, excised leaves of V. faba were attached to the surface of 1% agar gel containing nutrient solution, and test aphids were transferred onto the leaves. Excised leaves grew in size and weight on the agar medium. Fecundity, longevity, body size and developmental time to adulthood were compared between aphids reared using the agar-leaf method vs. those reared on V. faba seedlings under the same conditions. No significant difference was detected between the two treatments for any of the four parameters, suggesting that the aphids grew and reproduced on excised leaves as successfully as on V. faba seedlings. This method was also useful for inducing males and oviparous females at lower temperature and in short days. Therefore, the present study confirms the effectiveness of using excised leaves on agar and suggests that this method could be applied to the rearing of other aphids, phytophagous mites, leaf miners and leaf-gall formers.
Rights: This is the pre-peer reviewed version of the following article: Li, Yang; Akimoto, Shin-ichi (2018). Evaluation of an aphid-rearing method using excised leaves and agar medium. Entomological science, 21(1), 210-215., which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 秋元 信一

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