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Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation

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Title: Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation
Authors: Yamazaki, Tomohiro Browse this author
Souquere, Sylvie Browse this author
Chujo, Takeshi Browse this author
Kobelke, Simon Browse this author
Chong, Yee Seng Browse this author
Fox, Archa H. Browse this author
Bond, Charles S. Browse this author
Nakagawa, Shinichi Browse this author →KAKEN DB
Pierron, Gerard Browse this author
Hirose, Tetsuro Browse this author →KAKEN DB
Keywords: noncoding RNA
architectural RNA
nuclear bodies
paraspeckle
RNA-binding proteins
prion-like domain
phase separation
1,6-hexanediol
CRISPR/Cas9
haploid cell
Issue Date: 21-Jun-2018
Publisher: Cell Press
Journal Title: Molecular cell
Volume: 70
Issue: 6
Start Page: 1038
End Page: 1053
Publisher DOI: 10.1016/j.molcel.2018.05.019
Abstract: A class of long noncoding RNAs (lncRNAs) has architectural functions in nuclear body construction; however, specific RNA domains dictating their architectural functions remain uninvestigated. Here, we identified the domains of the architectural NEAT1 lncRNA that construct paraspeckles. Systematic deletion of NEAT1 portions using CRISPR/Cas9 in haploid cells revealed modular domains of NEAT1 important for RNA stability, isoform switching, and paraspeckle assembly. The middle domain, containing functionally redundant subdomains, was responsible for paraspeckle assembly. Artificial tethering of the NONO protein to a NEAT1_2 mutant lacking the functional subdomains rescued paraspeckle assembly, and this required the NOPS dimerization domain of NONO. Paraspeckles exhibit phase-separated properties including susceptibility to 1,6-hexanediol treatment. RNA fragments of the NEAT1_2 subdomains preferentially bound NONO/SFPQ, leading to phase-separated aggregates in vitro. Thus, we demonstrate that the enrichment of NONO dimers on the redundant NEAT1_2 subdomains initiates construction of phase-separated paraspeckles, providing mechanistic insights into lncRNA-based nuclear body formation.
Rights: © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/74758
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 廣瀬 哲郎

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