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Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice : Novel implication for vascular atrophy

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Title: Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice : Novel implication for vascular atrophy
Authors: Kishimoto, Ayuko Browse this author
Kimura, Shunsuke Browse this author →KAKEN DB
Nio-Kobayashi, Junko Browse this author →KAKEN DB
Takahashi-Iwanaga, Hiromi Browse this author →KAKEN DB
Park, Ah-Mee Browse this author
Iwanaga, Toshihiko Browse this author →KAKEN DB
Keywords: Galectin-3
Hyaloid vessels
Issue Date: Jul-2018
Publisher: Elsevier
Journal Title: Experimental eye research
Volume: 172
Start Page: 1
End Page: 9
Publisher DOI: 10.1016/j.exer.2018.03.024
PMID: 29596849
Abstract: The hyaloid vasculature constitutes a transitory system nourishing the internal structures of the developing eye, but the mechanism of vascular regression and its cell biological characteristics are not fully understood. The present study aimed to reveal the specificity of the hyaloid vessels by a systematic immunohistochemical approach for marker substances of myeloid cells and the extracellular matrix (ECM) in neonatal mice. Macrophages immunoreactive for F4/80, cathepsin D, and LYVE-1 gathered around the vasa hyaloidea propria (VHP), while small round cells in vascular lumen of VHP were selectively immunoreactive for galectin-3; their segmented nuclei and immunoreactivities for Ly-6G, CD11b, and myeloperoxidase indicated their neutrophilic origin. VHP possessed thick ECM and a dense pericyte envelope as demonstrated by immunostaining for laminin, type IV collagen, integrin β1, and NG2. The galectin-3+ cells loosely aggregated with numerous erythrocytes in the lumen of hyaloid vessels in a manner reminiscent of vascular congestion. Galectin-3 is known to polymerize and form a complex with ECM and NG2 as well as recruit leukocytes on the endothelium. Observation of galectin-3 KO mice implicated the involvement of galectin-3 in the regression of hyaloid vasculature. Since macrophages may play central roles including blocking of the blood flow and the induction of apoptosis in the regression, galectin-3+ neutrophils may play a supportive role in the macrophage-mediated involution of the hyaloid vascular system.
Rights: © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岩永 敏彦

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