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Acanthamoeba S13WT relies on its bacterial endosymbiont to backpack human pathogenic bacteria and resist Legionella infection on solid media

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/74841

Title: Acanthamoeba S13WT relies on its bacterial endosymbiont to backpack human pathogenic bacteria and resist Legionella infection on solid media
Authors: Okubo, Torahiko Browse this author →KAKEN DB
Matsushita, Mizue Browse this author
Nakamura, Shinji Browse this author →KAKEN DB
Matsuo, Junji Browse this author →KAKEN DB
Nagai, Hiroki Browse this author
Yamaguchi, Hiroyuki Browse this author →KAKEN DB
Issue Date: 1-Jun-2018
Publisher: John Wiley & Sons
Journal Title: Environmental microbiology reports
Volume: 10
Issue: 3
Start Page: 344
End Page: 354
Publisher DOI: 10.1111/1758-2229.12645
PMID: 29611898
Abstract: Soil-borne amoeba Acanthamoeba S13WT has an endosymbiotic relationship with an environmental Neochlamydia bacterial strain. However, regardless of extensive experiments in liquid media, the biological advantage of the symbiosis remained elusive. We therefore explored the role of the endosymbiont in predator-prey interactions on solid media. A mixed culture of the symbiotic or aposymbiotic amoebae and GFP-expressing Escherichia coli or Salmonella Enteritidis was spotted onto the centre of a LB or B-CYE agar plate preinoculated with a ring of mCherry-expressing Legionella pneumophila (Legionella wall'). The spread of the amoebae on the plate was assessed using a fluorescence imaging system or scanning electron microscopy. As a result, in contrast to the aposymbiotic amoebae, the symbiotic amoebae backpacked these GFP-expressing bacteria and formed flower-like fluorescence patterns in an anticlockwise direction. Other bacteria (Pseudomonas aeruginosa and Stenotrophomonas maltophilia), but not Staphylococcus aureus, were also backpacked by the symbiotic amoebae on LB agar, although lacked the movement to anticlockwise direction. Furthermore, in contrast to the aposymbiotic amoebae, the symbiotic amoebae backpacking the E. coli broke through the Legionella wall' on B-CYE agar plates. Thus, we concluded that Acanthamoeba S13WT required the Neochlamydia endosymbiont to backpack human pathogenic bacteria and resist Legionella infection on solid agar.
Rights: This is the peer reviewed version of the following article: Environmental Microbiology Reports, 10(3), 344-354, Jun. 2018, which has been published in final form at https://doi.org/10.1111/1758-2229.12645. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
URI: http://hdl.handle.net/2115/74841
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 博之

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