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Multipoint fluorescence correlation spectroscopy using spatial light modulator

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Title: Multipoint fluorescence correlation spectroscopy using spatial light modulator
Authors: Yamamoto, Johtaro Browse this author →KAKEN DB
Mikuni, Shintaro Browse this author →KAKEN DB
Kinjo, Masataka Browse this author →KAKEN DB
Issue Date: 1-Dec-2018
Publisher: Optical Society of America
Journal Title: Biomedical Optics Express
Volume: 9
Issue: 12
Start Page: 5881
End Page: 5890
Publisher DOI: 10.1364/BOE.9.005881
Abstract: A multipoint holographic fluorescence correlation spectroscope (MP-hFCS) was successfully developed. The validity of the MP-hFCS was demonstrated using diffusion measurements of fluorescent dye solutions and of fluorescent proteins in single cells. Furthermore, the successful detection of the nuclear transport of a green fluorescent protein-tagged glucocorticoid receptor a indicates the possibility of being able to monitor directional molecular transport using the MP-hFCS. This allows multipoint analysis of the intermolecular interactions and molecular transport in living cells. Finally, the MP-hFCS can achieve multipoint diffusion measurements with high spatial and time resolution while maintaining a high photon detection sensitivity. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Rights: ©2018 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
Type: article
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 金城 政孝

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