Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP)

Takehana, Kazuya; Kinjyo, Teruo; Nemoto, Manabu; Matsuno, Keita

doi:10.1292/jvms.18-0683


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Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP)

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/74932

Title:	Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP)
Authors:	Takehana, Kazuya Browse this author
	Kinjyo, Teruo Browse this author
	Nemoto, Manabu Browse this author
	Matsuno, Keita Browse this author →KAKEN DB
Keywords:	Acute hemorrhagic disease
	Asian elephants (Elephas maximus)
	Elephant endotheliotropic herpesvirus (EEHV)
	Loop-mediated isothermal amplification (LAMP)
	Polymerase chain reaction (PCR)
Issue Date:	Mar-2019
Publisher:	公益社団法人日本獣医学会
Journal Title:	Journal of veterinary medical science
Volume:	81
Issue:	3
Start Page:	504
End Page:	507
Publisher DOI:	10.1292/jvms.18-0683
PMID:	30700652
Abstract:	Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants (Elephas maximus). We employed loop-mediated isothermal amplification (LAMP) to develop a rapid and simple detection method for EEHV1 in blood. When used to test 21 clinical samples collected in Japan, the EEHV1 assay correctly identified one positive and 20 negative clinical samples. It was observed that when samples were spiked with synthetic DNA plasmids including EEHV1 polymerase gene, the detection limit of the LAMP assay was 101.2 copies/mu l and 100-fold higher than that of conventional PCR. These advantages of the LAMP assay for EEHV1 detection may facilitate better veterinary practices for treating elephants suffering from the acute disease.
Rights:	https://creativecommons.org/licenses/by-nc-nd/4.0/
Type:	article
URI:	http://hdl.handle.net/2115/74932
Appears in Collections:	国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc) 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 松野啓太

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