| Title: | Prognostic value of MAGEA4 in primary lung cancer depends on subcellular localization and p53 status |
| Authors: | Fujiwara-Kuroda, Aki Browse this author |
| Kato, Tatsuya Browse this author |
| Abiko, Takehiro Browse this author |
| Tsuchikawa, Takahiro Browse this author →KAKEN DB |
| Kyogoku, Noriaki Browse this author |
| Ichinokawa, Masaomi Browse this author |
| Tanaka, Kimitaka Browse this author |
| Noji, Takehiro Browse this author →KAKEN DB |
| Hida, Yasuhiro Browse this author →KAKEN DB |
| Kaga, Kichizo Browse this author →KAKEN DB |
| Matsui, Yoshiro Browse this author →KAKEN DB |
| Ikeda, Hiroaki Browse this author →KAKEN DB |
| Kageyama, Shinichi Browse this author →KAKEN DB |
| Shiku, Hiroshi Browse this author →KAKEN DB |
| Hirano, Satoshi Browse this author →KAKEN DB |
| Keywords: | melanoma antigen family A4 |
| p53 |
| non-small cell lung cancer |
| subcellular localization |
| tissue microarray |
| apoptosis |
| Issue Date: | Aug-2018 |
| Publisher: | Spandidos Publications |
| Journal Title: | International journal of oncology |
| Volume: | 53 |
| Issue: | 2 |
| Start Page: | 713 |
| End Page: | 724 |
| Publisher DOI: | 10.3892/ijo.2018.4425 |
| PMID: | 29901069 |
| Abstract: | Melanoma antigen family A4 (MAGEA4), a cancer/testis antigen, is overexpressed and is thus an immunotherapy target in various malignant tumors, including non-small cell lung cancer. However, whether MAGEA4 induces or inhibits the apoptosis of lung cancer cells remains controversial, as is its prognostic significance, particularly since there is no reliable method with which to detect MAGEA4 specifically. In this study, we optimized assay conditions to detect MAGEA4 based on cells transiently transfected with MAGEA genes, and found that MAGEA4 was expressed in four of eight non-small cell lung cancer cell lines, and in 25.4% of clinical lung cancer specimens. We also found that MAGEA4 overexpression decreased apoptosis, as measured by the levels of cleaved caspase-3 in stably transfected 293F cells. Notably, patients with nuclear MAGEA4, but not p53 expression exhibited a significantly poorer survival than those expressing both nuclear MAGEA4 and p53. Indeed, multivariate analysis identified nuclear MAGEA4 as an independent prognostic factor (P=0.0042), albeit only in the absence of p53. In this study, to the best of our knowledge, we are the first to demonstrate that the function and prognostic value of MAGEA4 depends on its subcellular localization and on the p53 status. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/75061 |
| Appears in Collections: | 北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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