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Oncolytic potential of an E4-deficient adenovirus that can recognize the stabilization of AU-rich element containing mRNA in cancer cells

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Title: Oncolytic potential of an E4-deficient adenovirus that can recognize the stabilization of AU-rich element containing mRNA in cancer cells
Authors: Yanagawa-Matsuda, Aya Browse this author →KAKEN DB
Mikawa, Yohei Browse this author
Habiba, Umma Browse this author
Kitamura, Tetsuya Browse this author →KAKEN DB
Yasuda, Motoaki Browse this author →KAKEN DB
Towfik-Alam, Mohammad Browse this author →KAKEN DB
Kitagawa, Yoshimasa Browse this author →KAKEN DB
Minowa, Kazuyuki Browse this author →KAKEN DB
Shindoh, Masanobu Browse this author →KAKEN DB
Higashino, Fumihiro Browse this author →KAKEN DB
Keywords: adenovirus
AU-rich element
Issue Date: Feb-2019
Publisher: Spandidos Publications
Journal Title: Oncology reports
Volume: 41
Issue: 2
Start Page: 954
End Page: 960
Publisher DOI: 10.3892/or.2018.6865
PMID: 30431137
Abstract: AU-rich elements (AREs) are RNA elements that enhance the rapid decay of mRNA. The fate of ARE-mRNA is controlled by ARE-binding proteins. HuR, a member of the embryonic lethal abnormal vision (ELAV) family of RNA-binding proteins, is involved in the export and stabilization of ARE-mRNA. In the vast majority of cancer cells, HuR constitutively relocates to the cytoplasm, resulting in the stabilization of ARE-mRNA. Previously, we described that the adenovirus gene product, E4orf6, which is necessary for virus replication, participates in ARE-mRNA export and stabilization. In the present study, we showed the oncolytic potential of E4orf6-deleted adenovirus dl355, which is expected to be replicated selectively in cancer cells. Virus production and cytolytic activity of dl355 were higher in cancer cells than in normal cells. HuR-depletion downregulated dl355 replication, demonstrating that ARE-mRNA stabilization is required for the production of this virus. Tumor growth was inhibited in nude mice by an intratumoral injection of dl355. Furthermore, dl355 had a stronger oncolytic effect than E1B55k-deleted adenovirus. These results indicate that dl355 has potential as an oncolytic adenovirus for a large number of cancers where ARE-mRNA is stabilized.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 東野 史裕

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