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Bioactive micropatterning of apatite immobilizing cell adhesion protein by laser-induced forward transfer with a shock absorber

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Title: Bioactive micropatterning of apatite immobilizing cell adhesion protein by laser-induced forward transfer with a shock absorber
Authors: Narazaki, Aiko Browse this author
Oyane, Ayako Browse this author
Komuro, Saki Browse this author
Kurosaki, Ryozo Browse this author
Kameyama, Tomoko Browse this author
Sakamaki, Ikuko Browse this author
Araki, Hiroko Browse this author
Miyaji, Hirofumi Browse this author →KAKEN DB
Issue Date: 1-Jul-2019
Publisher: Optical Society of America
Journal Title: Optical materials express
Volume: 9
Issue: 7
Start Page: 2807
End Page: 2816
Publisher DOI: 10.1364/OME.9.002807
Abstract: The additive patterning of apatite with good biocompatibility and osteoconductivity is a useful technique for the production and surface functionalization of biomaterials. We developed this technique through our laser-induced forward transfer (LIFT) process using a laser-absorbing sacrificial layer in combination with a shock-absorbing polydimethylsiloxane (PDMS) receiver. With the PDMS shock-absorbing function, even the brittle apatite and that immobilizing the cell adhesion protein fibronectin (Fn-apatite) were successfully transferred and micropatterned while maintaining their dense, filmy state. The laser pulse energy effect was investigated, leading to the optimum energy range just above the transfer threshold. The apatite and Fn-apatite micropatterns exhibited superior cytocompatibility compared to the PDMS surface, and could potentially be used for cellular micromanipulation. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Rights: © 2019 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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