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Real-Time Polarization-Resolved Imaging of Living Tissues Based on Two-Photon Excitation Spinning-Disk Confocal Microscopy
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| Title: | Real-Time Polarization-Resolved Imaging of Living Tissues Based on Two-Photon Excitation Spinning-Disk Confocal Microscopy |
| Authors: | Goto, Ai Browse this author | | Otomo, Kohei Browse this author →KAKEN DB | | Nemoto, Tomomi Browse this author →KAKEN DB |
| Keywords: | second harmonic generation | | polarization-resolved optical microscopy | | in vivo imaging | | collagen | | spinning-disk microscopy | | two-photon excitation fluorescence microscopy |
| Issue Date: | 16-Apr-2019 |
| Publisher: | Frontiers Media |
| Journal Title: | Frontiers in physics |
| Volume: | 7 |
| Start Page: | 56 |
| Publisher DOI: | 10.3389/fphy.2019.00056 |
| Abstract: | Laser scanning microscopy using high-peak-power ultrashort near infrared light pulses can visualize biological microstructures by utilizing non-linear optical processes, such as multi-photon excitation and sum frequency generation. Here we introduced a polarization-resolving detection methodology for a laser scanning microscopy system equipped with a spinning-disk confocal scanner. The developed system achieved high-speed intravital imaging of living tissues with resolving their signals to orthogonally polarized components. First, we applied the system to a liposomal vesicle labeled with the fluorescent lipophilic dye and con firmed the orientation map of the lipid bilayer. Next, by detecting polarization-resolved second harmonic generation signals, the structural orientations of the collagen fibers in fixed mouse tissues were visualized without exogenous or genetic fluorophore labeling. Finally, we demonstrated in vivo polarization-resolved second harmonic generation imaging of the collagen fibers in the mouse skeletal muscles at a 56 Hz temporal resolution. We expect that our developed methodology can achieve real-time visualization, thus, revealing the conformational changes of supramolecular structures in living animals. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/75162 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 大友 康平
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