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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Interaction of Nerve Growth Factor beta with Adiponectin and SPARC Oppositely Modulates its Biological Activity
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| Title: | Interaction of Nerve Growth Factor beta with Adiponectin and SPARC Oppositely Modulates its Biological Activity |
| Authors: | Okura, Yuu Browse this author | | Imao, Takeshi Browse this author | | Murashima, Seisuke Browse this author | | Shibata, Haruki Browse this author | | Kamikavwa, Akihiro Browse this author | | Okamatsu-Ogura, Yuko Browse this author | | Saito, Masayuki Browse this author | | Kimura, Kazuhiro Browse this author →KAKEN DB |
| Keywords: | adiponectin | | AMPK | | BIAcore | | extracellular signal-regulated kinase (ERK) | | matricellular proteins | | neuritogenesis | | NGF beta | | PC12 cells | | Secreted protein | | acidic and rich in cysteine (SPARC) |
| Issue Date: | 27-Mar-2019 |
| Publisher: | MDPI |
| Journal Title: | International Journal of Molecular Sciences |
| Volume: | 20 |
| Issue: | 7 |
| Start Page: | 1541 |
| Publisher DOI: | 10.3390/ijms20071541 |
| PMID: | 30934765 |
| Abstract: | Both adiponectin and secreted protein, acidic and rich in cysteine (SPARC) inhibit platelet-derived growth factor-BB (PDGF-BB)-induced and basic fibroblast growth factor (FGF2)-induced angiogenic activities through direct and indirect interactions. Although SPARC enhances nerve growth factor (NGF)-dependent neurogenesis, the physical interaction of NGF beta with adiponectin and SPARC remains obscure. Therefore, we first examined their intermolecular interaction by surface plasmon resonance method. NGF beta bound to immobilized SPARC with the binding constant of 59.4 nM, comparable with that of PDGF-BB (24.5 nM) but far less than that of FGF2 (14.4 mu M). NGF beta bound to immobilized full length adiponectin with the binding constant of 103 nM, slightly higher than those of PDGF-BB (24.3 nM) and FGF2 (80.2 nM), respectively. Treatment of PC12 cells with SPARC did not cause mitogen-activated protein kinase (MAPK) activation and neurite outgrowth. However, simultaneous addition of SPARC with NGF beta enhanced NGF beta-induced MAPK phosphorylation and neurite outgrowth. Treatment of the cells with adiponectin increased AMP-activated protein kinase (AMPK) phosphorylation but failed to induce neurite outgrowth. Simultaneous treatment with NGF beta and adiponectin significantly reduced cell size and the number of cells with neurite, even after silencing the adiponectin receptors by their siRNA. These results indicate that NGF beta directly interacts with adiponectin and SPARC, whereas these interactions oppositely regulate NGF beta functions. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/75164 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 和弘
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