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Two-photon STED nanoscopy realizing 100-nm spatial resolution utilizing high-peak-power sub-nanosecond 655-nm pulses

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Title: Two-photon STED nanoscopy realizing 100-nm spatial resolution utilizing high-peak-power sub-nanosecond 655-nm pulses
Authors: Ishii, Hirokazu Browse this author
Otomo, Kohei Browse this author →KAKEN DB
Hung, Jui-Hung Browse this author
Tsutsumi, Motosuke Browse this author →KAKEN DB
Yokoyama, Hiroyuki Browse this author
Nemoto, Tomomi Browse this author →KAKEN DB
Issue Date: 1-Jul-2019
Publisher: Optical Society of America
Journal Title: Biomedical Optics Express
Volume: 10
Issue: 7
Start Page: 3104
End Page: 3113
Publisher DOI: 10.1364/BOE.10.003104
Abstract: We developed two-photon excitation stimulated emission depletion (STED) nanoscopy using high-peak-power sub-nanosecond 655-nm pulses. The STED pulse exhibited ideal optical properties and sufficient pulse energy to realize a 70-nm spatial resolution in the compact setup With electrically controllable components. For biological applications, we screened suitable fluorescent dyes or proteins and realized the sub-100 nm spatial resolution imaging of presynaptic protein clusters in fixed primary cultured neurons Without severe photobleaching. We expect this method to enable visualization of ultrastructures and the cluster dynamics of biomolecules representing physiological functions in living cells and tissue. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Rights: © 2019 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
Type: article
URI: http://hdl.handle.net/2115/75168
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 根本 知己

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