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Two-photon STED nanoscopy realizing 100-nm spatial resolution utilizing high-peak-power sub-nanosecond 655-nm pulses
| Title: | Two-photon STED nanoscopy realizing 100-nm spatial resolution utilizing high-peak-power sub-nanosecond 655-nm pulses |
| Authors: | Ishii, Hirokazu Browse this author | | Otomo, Kohei Browse this author →KAKEN DB | | Hung, Jui-Hung Browse this author | | Tsutsumi, Motosuke Browse this author →KAKEN DB | | Yokoyama, Hiroyuki Browse this author | | Nemoto, Tomomi Browse this author →KAKEN DB |
| Issue Date: | 1-Jul-2019 |
| Publisher: | Optical Society of America |
| Journal Title: | Biomedical Optics Express |
| Volume: | 10 |
| Issue: | 7 |
| Start Page: | 3104 |
| End Page: | 3113 |
| Publisher DOI: | 10.1364/BOE.10.003104 |
| Abstract: | We developed two-photon excitation stimulated emission depletion (STED) nanoscopy using high-peak-power sub-nanosecond 655-nm pulses. The STED pulse exhibited ideal optical properties and sufficient pulse energy to realize a 70-nm spatial resolution in the compact setup With electrically controllable components. For biological applications, we screened suitable fluorescent dyes or proteins and realized the sub-100 nm spatial resolution imaging of presynaptic protein clusters in fixed primary cultured neurons Without severe photobleaching. We expect this method to enable visualization of ultrastructures and the cluster dynamics of biomolecules representing physiological functions in living cells and tissue. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
| Rights: | © 2019 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/75168 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 根本 知己
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