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Resistance to Oncogenic Transformation in Revertant R1 of Human ras-Transformed NIH 3T3 cells

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タイトル: Resistance to Oncogenic Transformation in Revertant R1 of Human ras-Transformed NIH 3T3 cells
著者: Kuzumaki, Noboru 著作を一覧する
Ogiso, Yoshifumi 著作を一覧する
Oda, Atsushi 著作を一覧する
Fujita, Hisakazu 著作を一覧する
Suzuki, Hiroaki 著作を一覧する
Sato, Chiharu 著作を一覧する
Mullauer, Leonhard 著作を一覧する
発行日: 1989年
出版者: American Society for Microbiology
誌名: Molecular and Cellular Biology
巻: 9
号: 5
開始ページ: 2258
終了ページ: 2263
抄録: A flat revertant, R1, was isolated from human activated c-Ha-ras-1 (hu-ac-Ha-ras) gene-transformed NIH 3T3 cells (EJ-NIH 3T3) treated with mutagens. R1 contained unchanged transfected hu-ac-Ha-ras DNA and expressed high levels of hu-ac-Ha-ras-specific mRNA and p21 protein. Transfection experiments revealed that NIH 3T3 cells could be transformed by DNA from R1 cells but R1 cells could not be retransformed by Kirsten sarcoma virus, DNA from EJ-NIH 3T3 cells, hu-ac-Ha-ras, v-src, v-mos, simian virus 40 large T antigen, or polyomavirus middle T antigen. Somatic cell hybridization studies showed that R1 was not retransformed by fusion with NIH 3T3 cells and suppressed anchorage independence of EJ-NIH 3T3 and hu-ac-Ha-ras gene-transformed rat W31 cells in soft agar. These results suggest that the reversion and resistance to several oncogenes in R1 is due not to cellular defects in the production of the transformed phenotype but rather to enhancement of cellular mechanisms that suppress oncogenic transformation.
Rights: Copyright (c) 1989 American Society for Microbiology
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 葛巻 暹


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