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Investigation of mRNA expression changes associated with field exposure to DDTs in chickens from KwaZulu-Natal, South Africa

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/75302

Title: Investigation of mRNA expression changes associated with field exposure to DDTs in chickens from KwaZulu-Natal, South Africa
Authors: Thompson, Lesa A. Browse this author
Ikenaka, Yoshinori Browse this author
Darwish, Wageh S. Browse this author
Yohannes, Yared B. Browse this author
van Vuren, Johan J. Browse this author
Wepener, Victor Browse this author
Smit, Nico J. Browse this author
Assefa, Atnafu G. Browse this author
Tharwat, Ahmed Browse this author
Eldin, Walaa Fathy Saad Browse this author
Nakayama, Shouta M. M. Browse this author
Mizukawa, Hazuki Browse this author
Ishizuka, Mayumi Browse this author →KAKEN DB
Issue Date: 11-Oct-2018
Publisher: PLOS
Journal Title: PLoS ONE
Volume: 13
Issue: 10
Start Page: e0204400
Publisher DOI: 10.1371/journal.pone.0204400
PMID: 30307967
Abstract: The objective of this study was to identify potential mRNA expression changes in chicken livers associated with environmental exposure to dichloro-diphenyl-trichloroethane (DDT) and its metabolites (DDTs). In particular, we focused on genes relating to the immune system and metabolism. We analyzed liver samples from free-ranging chickens in KwaZulu-Natal, South Africa, for contamination by DDTs. This area predominantly uses DDT in its malaria control program, and homes are sprayed annually with the pesticide. Genes relating to the immune system and metabolism were selected as potential genetic biomarkers that could be linked to higher contamination with DDTs. RT-qPCR analysis on 39 samples showed strong correlations between DDTs contamination and mRNA expression for the following genes: AvBD1, AvBD2, AvBD6 and AvBD7(down-regulated), and CYP17, ELOVL2 and SOLE (up-regulated). This study shows for the first time interesting and significant correlations between genetic material collected from environmentally-exposed chickens and mRNA expression of several genes involved in immunity and metabolism. These findings show the usefulness of analysis on field samples from a region with high levels of environmental contamination in detecting potential biomarkers of exposure. In particular, we observed clear effects from DDT contamination on mRNA expression of genes involved in immune suppression, endocrine-disrupting effects, and lipid dysregulation. These results are of interest in guiding future studies to further elucidate the pathways involved in and clinical importance of toxicity associated with DDT exposure from contaminated environments, to ascertain the health risk to livestock and any subsequent risks to food security for people.
Rights: http://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/75302
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石塚 真由美

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