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Differences in interleukin-1β release-inducing activity of Candida albicans toward dendritic cells and macrophages.

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Title: Differences in interleukin-1β release-inducing activity of Candida albicans toward dendritic cells and macrophages.
Authors: Hasebe, A Browse this author →KAKEN DB
Saeki, A Browse this author →KAKEN DB
Yoshida, Y Browse this author →KAKEN DB
Shibata, KI Browse this author →KAKEN DB
Keywords: Interleukin-1β
Candida albicans
Dendritic cells
Macrophages
Issue Date: Sep-2018
Journal Title: Archives of oral biology
Volume: 93
Start Page: 115
End Page: 125
Publisher DOI: 10.1016/j.archoralbio.2018.06.004
PMID: 29894908
Abstract: Objective: The purpose of this study is to elucidate differences in the mechanism of the IL-1β release-inducing activity of Candida albicans toward dendritic cells and macrophages because IL-1β is one of the proinflammatory cytokines which is crucial in host defense against candidiasis.Design: Two C. albicans strains were used in this study. One strain is uridineauxotrophic (CAI4) that needs uridine to grow and form hyphae, and another is a strain without any specific auxotrophy (pACT1-GFP), which forms hyphae naturally by culturing with serum components. Murine macrophage and dendritic cell lines were primed with LPS and then stimulated with C. albicans CAI4 or pACT1-GFP. Results: Both strains of C. albicans induced IL-1β release from dendritic cells, and C. albicans pACT1-GFP induced IL-1β release but CAI4 induced little amounts in macrophages. These differences were suggested to be due to the difference in the amount of extracellular ATP released in the cell culture supernatants induced by C. albicans CAI4 or pACT1-GFP. For induction of IL-1β release from both macrophages and dendritic cells by C. albicans, direct contacts of the microbes with cells were required. In addition, macrophages required morphological change of C. albicans from yeast to hyphae for induction of IL-1β release, whereas dendritic cells did not require it. Dead C. albicans could induce IL-1β release from dendritic cells, but could not from macrophages. Conclusions: There are different mechanisms by which C. albicans induces IL-1β release from dendritic cells and macrophages.
Rights: © <2018>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/75324
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷部 晃

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