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Optimization of yield and quality of agar polysaccharide isolated from the marine red macroalga Pyropia yezoensis

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Title: Optimization of yield and quality of agar polysaccharide isolated from the marine red macroalga Pyropia yezoensis
Authors: Sasuga, Keiji Browse this author
Yamanashi, Tomoya Browse this author
Nakayama, Sigeru Browse this author
Ono, Syuetsu Browse this author
Mikami, Koji Browse this author →KAKEN DB
Keywords: Agar
Physical property
Extraction optimization
Macroalga
Red seaweed
Pyropia yezoensis
Issue Date: Sep-2017
Journal Title: Algal Research
Volume: 26
Start Page: 123
End Page: 130
Publisher DOI: 10.1016/j.algal.2017.07.010
Abstract: The marine red seaweed Pyropia yezoensis is grown on a large scale in Japan using mariculture for production of nori sheets. Here, we optimized the isolation of agar from P. yezoensis and evaluated its yield, physical properties, and product applications. An alkali pre-treatment of seaweeds before agar extraction increased the weight-average molecular weight of the agar and promoted the conversion of L-galactose sulfate to 3,6-anhydrogalactose, which in turn reduced the sulfate contents, thus altering the gel strength of the agar. This ability to adjust agar quality by altering the alkali concentration during material pre-treatment allowed production of agar with properties similar to those of agar from the red seaweed Gelidium sp., which is widely used for industrial agar applications. We demonstrate the suitability of P. yezoensis agar in production of solid plates for bacterial growth. In addition, the P. yezoensis agar was particularly useful as a gel material, with the capacity for excellent size separation of DNA by electrophoresis even without agarose purification, because of quite low contents of sulfate. These findings support the applicability and usefulness of P. yezoensis for agar production. The established large-scale cultivation methods for P. yezoensis can generate sufficient biomass to produce agar to support medical and biological studies.
Rights: © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/75361
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 三上 浩司

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