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Analysis of time-course drug response in rat cardiomyocytes cultured on a pattern of islands
| Title: | Analysis of time-course drug response in rat cardiomyocytes cultured on a pattern of islands |
| Authors: | Okumura, Sho Browse this author | | Hirano, Yu Browse this author →KAKEN DB | | Maki, Yoshiyuki Browse this author | | Komatsu, Yasuo Browse this author →KAKEN DB |
| Issue Date: | 7-Sep-2018 |
| Publisher: | Royal Society of Chemistry |
| Journal Title: | Analyst |
| Volume: | 143 |
| Issue: | 17 |
| Start Page: | 4083 |
| End Page: | 4089 |
| Publisher DOI: | 10.1039/c8an01033a |
| Abstract: | We previously reported the kinetics analysis of cardiomyocyte beating using scanning electrochemical microscopy (SECM). In this study, a stage-top incubator and a capillary micropipette (MP) for delivering drugs were assembled with an SECM instrument, and the responses of rat cardiomyocytes were analyzed under a culture environment after drug stimulation. When adenosine triphosphate (ATP) was delivered to synchronously beating cardiomyocytes, the beating acceleration effect of ATP was counteracted by the synchronously beating network in the culture dish. In contrast, cardiomyocytes cultured on a pattern of islands in a culture dish showed fluctuations in the duration of beating upon the addition of ATP. We also examined the effect of the cardiotoxic agent astemizole on cardiomyocytes and successfully detected motion fluctuations. Therefore, drug stimulation via MPs and beating measurement by SECM are effective routes for the evaluation of drug candidates through the analysis of time-course beating motion fluctuations of the cardiomyocytes. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/75436 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 平野 悠
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