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Biochemical analysis of human tRNA(His) guanylyltransferase in mitochondrial tRNA(His) maturation

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Title: Biochemical analysis of human tRNA(His) guanylyltransferase in mitochondrial tRNA(His) maturation
Authors: Nakamura, Akiyoshi Browse this author
Wang, Daole Browse this author
Komatsu, Yasuo Browse this author →KAKEN DB
Keywords: Transfer RNA
RNA modification
Mitochondria
Guanylyltransferase
Issue Date: 10-Sep-2018
Publisher: Elsevier
Journal Title: Biochemical and biophysical research communications
Volume: 503
Issue: 3
Start Page: 2015
End Page: 2021
Publisher DOI: 10.1016/j.bbrc.2018.07.150
Abstract: Mitochondria contain their own protein synthesis machinery, which includes mitochondrial tRNA maturation. It has been suggested that mammalian mitochondrial tRNA(His) (mtRNA(His)) is matured by post-transcriptional addition of guanosine at the 1 position (G(-1)), which serves as an identity element for mitochondrial histidyl-tRNA synthetase. However, the exact maturation process of mammalian mtRNA(His) remains unclear. In cytoplasmic tRNA(His) (ctRNA(His)) maturation, tRNA(His) guanylyltransferase (Thg1) adds a GTP onto the 5'-terminal of ctRNA(His) and then removes the 5'-pyrophosphate to yield the mature 5'-monophospholylated G(-1)-ctRNA(His) (pG(-1)-ctRNA(His)). Although mammalian Thg1 is localized to both the cytoplasm and mitochondria, it remains unclear whether mammalian Thg1 plays a role in mtRNA(His) maturation in mitochondria. Here, we demonstrated that human Thg1 (hThg1) catalyzes the G(-1) addition reaction for both human ctRNA(His) and mtRNA(His) through recognition of the anticodon. While hThg1 catalyzed consecutive GTP additions to mtRNA(His) in vitro, it did not exhibit any activity toward mature pG(-1)-mtRNA(His). We further found that hThg1 could add a GMP directly to the 5'-terminal of mtRNA(His) in a template-dependent manner, but fungal Thg1 could not. Therefore, we hypothesized that acceleration of the pyrophosphate removal activity before or after the G(-1) addition reaction is a key feature of hThg1 for maintaining a normal 5'-terminal of mtRNA(His) in human mitochondria. This study provided a new insight into the differences between tRNA(His) maturation in the cytoplasm and mitochondria of humans. (C) 2018 Elsevier Inc. All rights reserved.
Rights: ©2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/75437
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 中村 彰良

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